Optimal conditions for storing blood samples for assessing the hemocompatibility of medical devices in vitro

The purpose of the work is to establish optimal storage conditions for heparinized human whole blood before launching it into dynamic in vitro test systems of artificial blood flow to assess the hemocompatibility of medical devices. To conduct the study, blood was collected from donors in a volume of 150 ml using a butterfly needle with a luer-adapter, a catheter and a syringe filled with heparin (1.5 IU/ml). Subsequently, heparinized blood was transferred into test tubes and kept in a thermostat at temperatures of 20, 25, 30 and 37 oC for 40, 50 and 60 minutes, simulating room temperature and human body temperature. As a control sample, solid blood obtained immediately after its fence in donors was used, with the addition of heparin. To study the effect of time and temperature of blood storage on its activation, a number of hematological and immunoenzyme parameters were studied. Storing whole blood at room temperature initiated activation of platelets and coagulation mechanisms within 60 minutes. The effect of elevated temperatures of 30 and 37 oC was characterized by a statistically significant increase in the content of indicators such as beta-thromboglobulin (by 2.2–2.3 times), thromboxane B2 (by 2.0–2.1 times), prothrombin F1+2 (by 1.6– 1.8 times) and thrombin-antithrombin complex III (by 1.8–2.0 times) after 40 minutes of storage. It was experimentally established that to assess the hem-proposal of medical products with optimal storage conditions for solid heparinized blood of a person is a storage time of not more than 50 minutes and room temperature of 20–25 °C. The data obtained will help improve the reliability of assessing the blood compatibility of medical devices and, as a result, reduce the risk of adverse consequences for patients.