Key result
Mechanical stretch and serum availability interact differently to alter skeletal alpha-actin expression in cultured cardiac and skeletal muscle cells.
Population
Cultured chicken primary skeletal myoblasts and cardiac myocytes plated on collagenized Silastic membranes
Comparison
Mechanical stretch and varying serum availability vs Unstretched cells and alternative serum conditions
Design
Preclinical
Authors
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Cell culture findings on actin regulation require in vivo validation; leaves open distinct mechanosensitive pathways in cardiac versus skeletal muscle.
Mechanical stretch and serum availability differentially regulate skeletal alpha-actin gene expression in cardiac versus skeletal muscle cells through distinct DNA binding elements.
Carson et al. (1998) studied this question. Mechanical stretch and serum availability vs. Unstretched cells and alternative serum concentrations was evaluated on Skeletal alpha-actin (SkA) mRNA and promoter activity. Mechanical stretch and serum availability interact differently to alter skeletal alpha-actin expression in cultured cardiac and skeletal muscle cells.
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