July 26, 1999Biochemical Journal

Specific inhibition of skeletal α-actin gene transcription by applied mechanical forces through integrins and actin

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Population

Rat-2 fibroblasts and L8 cells transiently transfected with plasmids containing 5′-regulatory regions of the…

Comparison

Constant, perpendicular mechanical force applied… vs Unstressed cells and cells with Rous sarcoma…

Design

Preclinical

Follow-up

4 hours

Authors

ALApril M. LewUniversity of Toronto MGMichael GlogauerSt. Michael's Hospital CMChristopher A. McCullochUniversity of Toronto

Discussion

Member takes

Implication

Force inhibits skA in vitro; leaves open cytoskeletal-p38 regulation of actin in cardiac remodeling.

Structured PICO

Population

Rat-2 fibroblasts and L8 cells transiently transfected with plasmids containing 5′-regulatory regions of the skeletal α-actin (skA) gene fused to luciferase coding sequences

Intervention

Constant, perpendicular mechanical force (0.2 pN/μm2) applied using a collagen-magnetic bead model for 4 hours

Comparator

Unstressed cells (implied) and cells with Rous sarcoma virus promoter

Outcome

skA promoter activity and skA protein levelssurrogate

Applied mechanical forces inhibit skeletal α-actin gene transcription through a mechanism requiring an intact actin cytoskeleton and p38 kinase activation.

Cite This Study

Lew et al. (1999) studied this question.

synapsesocial.com/papers/6a6fba62f44fa9f079dd33b3 https://doi.org/10.1042/bj3410647

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