Key result
Substitution of the AG dinucleotide at nucleotides 34 and 35 of HCV-N with GA restored translational activity to that of HCV-H, indicating an inhibitory RNA-RNA interaction flanking the IRES.
Population
In vitro and in vivo models studying the 5' nontranslated RNA of Hepatitis C Virus genotypes 1a and 1b
Comparison
Mutational analysis including substitution of AG… vs Wild-type HCV-N 5'NTR
Design
Preclinical
Authors
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Identifies inhibitory RNA interaction in HCV 1b; extends IRES biology but hypothesis-generating for therapeutic targeting.
The study identifies an RNA-RNA interaction involving the 5'NTR and capsid-coding sequences flanking the IRES that is responsible for reduced IRES activity in HCV genotype 1b.
Honda et al. (1999) studied Hepatitis C Virus. Substitution of AG dinucleotide sequence at nt 34 and 35 with GA vs. Wild-type HCV-N 5'NTR was evaluated on Translational activity. Substitution of the AG dinucleotide at nucleotides 34 and 35 of HCV-N with GA restored translational activity to that of HCV-H, indicating an inhibitory RNA-RNA interaction flanking the IRES.
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