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October 1, 1971The Journal of Cell BiologyOpen Access

Sites of Lipoprotein Lipase Activity in Adipose Tissue Perfused With Chylomicrons

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Population

Rat parametrial adipose tissue and gelatin blocks containing postheparin plasma and chylomicrons

Comparison

Perfusion with chylomicrons and incubation in… vs Incubation with 0.004 M diethyl paranitrophenyl…

Design

Preclinical

Authors

EBE. Joan Blanchette‐MackieNational Institutes of HealthRSRobert O. ScowNational Institutes of Health

Discussion

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Implication

Should not change clinical lipid management; extends LPL localization data in animal adipose tissue.

Structured PICO

P
Population
Rat parametrial adipose tissue and gelatin blocks containing postheparin plasma and chylomicrons
I
Intervention
Perfusion with chylomicrons and incubation in 0.035 M CaCl(2)-0.1 M Tris medium (pH 8.3) at 38 degrees C
C
Comparator
Incubation with 0.004 M diethyl paranitrophenyl phosphate (E-600) as an inhibitor
O
Outcome
Sites of lipoprotein lipase activity (hydrolysis of chylomicron triglycerides) assessed by electron microscopysurrogate

This preclinical study demonstrates that plasma glycerides are hydrolyzed by lipoprotein lipase within capillary endothelial cells and the subendothelial space of adipose tissue.

Cite This Study

Blanchette‐Mackie et al. (1971) studied this question.

synapsesocial.com/papers/6a2076c204d2adee4bf48cb6https://doi.org/10.1083/jcb.51.1.1
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Also Consider

Synapse has enriched 3 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1A SIMPLIFIED LEAD CITRATE STAIN FOR USE IN ELECTRON MICROSCOPY1965 · 4,623 citations
  2. 2Effect of nutrition on activity and release of lipase from rat adipose tissue1959 · 257 citations
  3. 3ADIPOSE TISSUE1964 · 208 citations