Key result
The addition of LDL cholesterol to the perfusion medium at concentrations exceeding 4.0 mg/ml decreased the maximal removal rate of triglycerides by rat heart lipoprotein lipase to less than half.
Population
Isolated hearts from male Sprague-Dawley rats (350-400g, >10 weeks old) after an overnight fast.
Comparison
Perfusion with 3H labeled human VLDL or rat… vs Perfusion without LDL cholesterol.
Design
Preclinical
Follow-up
20-30 minutes
Authors
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High LDL may inhibit rat cardiac LPL activity; leaves open relevance to human triglyceride metabolism and atherosclerosis.
Human VLDL is an active substrate for rat heart lipoprotein lipase, and its activity is inhibited by abnormally high LDL cholesterol concentrations.
Wada et al. (1979) studied this question. Low Density Lipoprotein (LDL) cholesterol vs. Medium without LDL or lower LDL concentration was evaluated on Maximal removal rate of triglycerides (Vmax). The addition of LDL cholesterol to the perfusion medium at concentrations exceeding 4.0 mg/ml decreased the maximal removal rate of triglycerides by rat heart lipoprotein lipase to less than half.
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