Differences in the metabolism of very-low-density lipoproteins by isolated beating-heart cells and the isolated perfused rat heart. Evidence for collagenase-released extracellular lipoprotein lipase
Key result
Lipoprotein lipase responsible for VLD lipoprotein triacylglycerol hydrolysis is predominantly located externally to heart muscle cells and is released by bacterial collagenase perfusion.
Population
Intact isolated beating-heart cells and isolated perfused rat heart from starved animals
Design
Preclinical
Authors
Discussion
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Member takes
Implication
Indicates extracellular cardiac LPL in animals; leaves open its role in human VLDL metabolism.
Structured PICO
P
Population
Intact isolated beating-heart cells and isolated perfused rat heart from starved animals
I
Intervention
Perfusion with bacterial collagenase
O
Outcome
Metabolism of 14C-labelled VLD lipoproteinsurrogate
The study demonstrates that lipoprotein lipase, which hydrolyzes VLDL, is located extracellularly in the heart and can be released by collagenase.
Cite This Study
Rajaram et al. (1980) studied this question. Collagenase perfusion was evaluated on Metabolism of VLD lipoproteins and lipoprotein lipase activity. Lipoprotein lipase responsible for VLD lipoprotein triacylglycerol hydrolysis is predominantly located externally to heart muscle cells and is released by bacterial collagenase perfusion.