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This study investigates how quinidine affects action potentials and ionic currents in canine ventricular myocytes.

February 1, 1988Circulation ResearchOpen Access

Effects of quinidine on action potentials and ionic currents in isolated canine ventricular myocytes.

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Population

Isolated canine ventricular myocytes (collagenase treatment produced 40-60% healthy cells)

Comparison

Quinidine (5-20 microM) vs Control (baseline/washout)

Design

Preclinical

Authors

JSJoseph J. SalataMerck & Co., Inc., Rahway, NJ, USA (United States)JWJ. Andrew WasserstromElectrophysiology

Discussion

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Implication

Quinidine's multi-ion channel effects in canine myocytes warrant clinical caution; leaves open human translation and arrhythmia mechanism studies.

Key Points

This study investigates how quinidine affects action potentials and ionic currents in canine ventricular myocytes.
Isolated canine ventricular myocytes were treated with collagenase to analyze cell health.
Microelectrodes measured action potentials; a discontinuous single microelectrode voltage clamp was used for ionic currents.
Quinidine was tested at concentrations between 5-20 microM to determine its impact on various ionic currents.
Quinidine decreased maximum rate (Vmax) and duration of action potentials, with recovery observed after washout except for prolonged action potential duration compared to control.
A significant reduction in steady-state outward current was noted across three different voltage clamp protocols.
Quinidine caused a reversible decrease in peak calcium current and affected both late sodium and calcium currents, indicating complex modulation of ionic currents.

Structured PICO

Population

Isolated canine ventricular myocytes (collagenase treatment produced 40-60% healthy cells)

Intervention

Quinidine (5-20 microM)

Comparator

Control (baseline/washout)

Outcome

Transmembrane action potentials and ionic currents (Vmax, amplitude, overshoot, duration, steady-state outward current, peak calcium current, late inward currents)surrogate

Quinidine alters action potential configuration in canine ventricular myocytes through multiple actions on both inward and outward ionic currents.

Cite This Study

Salata et al. (1988) studied this question.

synapsesocial.com/papers/6a20799014cb6ef00ff48d51 https://doi.org/10.1161/01.res.62.2.324

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Implication

Quinidine's multi-ion channel effects in canine myocytes warrant clinical caution; leaves open human translation and arrhythmia mechanism studies.

Effects of quinidine on action potentials and ionic currents in isolated canine ventricular myocytes.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Effects of quinidine on action potentials and ionic currents in isolated canine ventricular myocytes.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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