July 1, 1989The Journal of PhysiologyOpen Access

Single inwardly rectifying potassium channels in cultured muscle cells from rat and mouse.

View Full Paper

Ask AI

Bookmark

Population

Myotubes derived from newborn rats or from a murine, clonal myoblast cell line

Design

Preclinical

Authors

HMHisashi MatsudaKanazawa University PSPeter StanfieldGoogle (United States)

Discussion

Member takes

Implication

Caution against extrapolating rodent myotube Na+ block to human cardiac Kir currents; leaves open species- and tissue-specific validation.

Structured PICO

Population

Myotubes derived from newborn rats or from a murine, clonal myoblast cell line

Intervention

Cell-attached configuration patch clamp with varying pipette solutions (150 mM-K+ or 40 mM-K+/200 mM-Na+) and voltage changes

Outcome

Single-channel conductance, open probability, and channel kinetics (lifetimes of openings and closed states)surrogate

Inwardly rectifying K+ channels in rat and mouse myotubes exhibit voltage-dependent block by external Na+ during hyperpolarization, explaining the inactivation of macroscopic inward currents.

Cite This Study

Matsuda et al. (1989) studied this question.

synapsesocial.com/papers/6a209447bdfec2d08e8a30fa https://doi.org/10.1113/jphysiol.1989.sp017679

View Full Paper

Ask AI

Bookmark

Implication

Caution against extrapolating rodent myotube Na+ block to human cardiac Kir currents; leaves open species- and tissue-specific validation.

Single inwardly rectifying potassium channels in cultured muscle cells from rat and mouse.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Single inwardly rectifying potassium channels in cultured muscle cells from rat and mouse.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider