Key result
Deac-aminoATP is an excellent fluorescent substrate for studying myosinV-S1 kinetics, revealing an ordered product dissociation mechanism where phosphate precedes ADP.
Population
Single headed myosinV-S1
Comparison
deac-aminoATP (3'--3'-deoxyadenosine-5'-triphosph… vs ATP
Design
Preclinical
Authors
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No immediate clinical implications; extends fluorescent tools for myosin kinetics research.
Deac-aminoATP is an excellent fluorescent substrate for studying the kinetic mechanism of myosinV-S1, revealing an ordered mechanism of product dissociation where phosphate precedes ADP.
Forgács et al. (2006) studied this question. deac-aminoATP vs. ATP was evaluated on ATP hydrolysis mechanism and kinetics. Deac-aminoATP is an excellent fluorescent substrate for studying myosinV-S1 kinetics, revealing an ordered product dissociation mechanism where phosphate precedes ADP.
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