Key result
ADP competes with ATP to inhibit myosin V ATPase rate, complicating kinetic analysis.
Population
Myosin V (single-headed and two-headed molecules)
Design
Preclinical
Authors
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Assay conditions for myosin V must account for ADP inhibition; leaves open refined protocols for accurate motor protein kinetics.
This study highlights that ADP product inhibition complicates the measurement of steady-state ATPase rates in myosin V, explaining inconsistencies in previous kinetic studies.
Cruz et al. (2000) studied this question. ADP vs. ATP was evaluated on steady-state ATPase rate. Under standard assay conditions, ADP competes with ATP, resulting in product inhibition of the myosin V ATPase rate and complicating kinetic analysis.
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