What question did this study set out to answer?

This research investigates how ADP and AMP-PNP bind to myosin subfragment 1 and actosubfragment 1.

March 16, 1982Biochemistry

Transient kinetics of ADP and AMP-PNP binding to subfragment 1 and actosubfragment 1

View Full Paper

Ask AI

Bookmark

Population

Myosin subfragment 1 (SF-1) and actosubfragment 1 (acto-SF-1)

Comparison

Binding of nonhydrolyzable nucleotides adenosine… vs Adenosine 5'-triphosphate (ATP) binding

Design

Preclinical

Authors

KTKathleen M. TrybusUniversity of Vermont ETEdwin W. TaylorOldham Council

Discussion

Member takes

Implication

Preclinical result warrants no practice change; extends molecular models of actomyosin kinetics and leaves cardiac translation open.

Key Points

This research investigates how ADP and AMP-PNP bind to myosin subfragment 1 and actosubfragment 1.
Reinvestigated the binding kinetics of ADP and AMP-PNP to SF-1 and acto-SF-1
Observed fluorescence transitions during nucleotide binding under varying ionic strengths
Analyzed at least three complexes necessary for explaining kinetic behaviors.
Fluorescence transitions differed significantly between ADP and AMP-PNP binding to SF-1
Under high ionic strength, AMP-PNP binding to acto-SF-1 showed early fluorescence changes before dissociation
Conformational changes were observed prior to the nucleotide-induced dissociation of actomyosin.

Structured PICO

Population

Myosin subfragment 1 (SF-1) and actosubfragment 1 (acto-SF-1)

Intervention

Binding of nonhydrolyzable nucleotides adenosine 5'-diphosphate (ADP) and adenosine 5'-(beta, gamma-imidotriphosphate) (AMP-PNP)

Comparator

Adenosine 5'-triphosphate (ATP) binding

Outcome

Kinetics of binding (rates and amplitudes of fluorescence transitions)surrogate

This basic science study demonstrates that a conformational change precedes the nucleotide-induced dissociation of actomyosin.

Cite This Study

Trybus et al. (1982) studied this question.

synapsesocial.com/papers/6a20a8389ca8a48788f36f3e https://doi.org/10.1021/bi00535a028

View Full Paper

Ask AI

Bookmark

Transient kinetics of ADP and AMP-PNP binding to subfragment 1 and actosubfragment 1

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Transient kinetics of ADP and AMP-PNP binding to subfragment 1 and actosubfragment 1

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider