What is the clinical evidence from this study?

Study design: Observational. Population: Myocarditis (n=163). Intervention: CD45RO immunohistochemistry. Primary outcome: Incidence of myocarditis.

What question did this study set out to answer?

To investigate T lymphocyte counts in myocardium using CD45RO immunohistochemistry and determine myocarditis incidence.

February 1, 2000Journal of Clinical PathologyOpen Access

Necropsy diagnosis of myocarditis: a retrospective study using CD45RO immunohistochemistry

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Key result

CD45RO immunohistochemistry of 163 routine necropsies revealed a 0.6% incidence of myocarditis, supporting a threshold of ≥14 lymphocytes/mm2 for diagnosis.

Why the study?

Does CD45RO immunohistochemistry improve the quantification of T lymphocytes and diagnosis of myocarditis in routine necropsy myocardial sections?

Population

Myocardial sections from 163 routine hospital necropsies

Comparison

CD45RO immunohistochemistry staining vs Routine light microscopy and clinical context

Design

Cohort

Authors

KFK M FeeleyRoyal Hallamshire Hospital JHJonathan HarrisSaint Barnabas Medical Center SSS K SuvarnaLeibniz University Hannover

Discussion

Member takes

Implication

Supports CD45RO IHC for necropsy myocarditis confirmation; leaves open prospective validation of the ≥14 lymphocytes/mm² threshold.

Key Points

To investigate T lymphocyte counts in myocardium using CD45RO immunohistochemistry and determine myocarditis incidence.
Analyzed myocardial sections from 163 routine hospital necropsies stained with CD45RO.
Counted positive lymphocytes per mm2 and correlated with H/E opinion and clinical context.
0.6% incidence of myocarditis found in the series.
Majority (143) had low counts of 0-3 CD45RO positive lymphocytes/mm2.
5 cases showed high counts (14 or more), with one confirmed case of active myocarditis.

Study Design

Type

Observational (n=163)

Structured PICO

Does CD45RO immunohistochemistry improve the quantification of T lymphocytes and diagnosis of myocarditis in routine necropsy myocardial sections?

Population

163 routine hospital necropsies evaluated for myocardial T lymphocyte counts using CD45RO immunohistochemistry.

Exposure

CD45RO immunohistochemistry staining

Comparator

Routine light microscopy (H/E staining) and clinical context

Outcome

Number of CD45RO positive lymphocytes/mm2 and incidence of myocarditissurrogate

CD45RO immunohistochemistry is a reliable method for confirming myocarditis at necropsy, supporting the 1997 ISFC task force threshold of ≥14 lymphocytes/mm2 for diagnosis.

Cite This Study

Feeley et al. (2000) conducted an observational in Myocarditis (n=163). CD45RO immunohistochemistry was evaluated on Incidence of myocarditis. CD45RO immunohistochemistry of 163 routine necropsies revealed a 0.6% incidence of myocarditis, supporting a threshold of ≥14 lymphocytes/mm2 for diagnosis.

synapsesocial.com/papers/6a20b0a79e00afa23b234477 https://doi.org/10.1136/jcp.53.2.147

Topics

Myocarditis

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Necropsy diagnosis of myocarditis: a retrospective study using CD45RO immunohistochemistry

Discussion

Member takes

Implication

Key Points

Study Design

Structured PICO

Cite This Study

Topics

Necropsy diagnosis of myocarditis: a retrospective study using CD45RO immunohistochemistry

Discussion

Member takes

Implication

Key Points

Study Design

Structured PICO

Cite This Study

Also Consider

Topics

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