The study successfully characterized and purified the lupus antigen La from HeLa cells, identifying multiple isoelectric forms.
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La antigen microheterogeneity observed in vitro; leaves open its pathogenic role in human autoimmunity.
HeLa cell La antigen, an RNA-binding protein, was characterized by using two-dimensional gel electrophoresis. Eight isoelectric forms (pI 6 to 7) were observed, many containing phosphate. An in vitro translation product similar in size and antigenicity was identified. The HeLa cell protein purified by using an assay based on ribonucleoprotein reconstitution with adenovirus VA RNAI also comprised several isoelectric forms.
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Francoeur et al. (1985) studied this question.
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