The study successfully characterized and purified the Lupus Antigen La from HeLa cells, identifying multiple isoelectric forms.
La antigen isoform diversity remains experimental; extends molecular characterization but leaves open roles in lupus pathogenesis.
HeLa cell La antigen, an RNA-binding protein, was characterized by using two-dimensional gel electrophoresis. Eight isoelectric forms (pI 6 to 7) were observed, many containing phosphate. An in vitro translation product similar in size and antigenicity was identified. The HeLa cell protein purified by using an assay based on ribonucleoprotein reconstitution with adenovirus VA RNAI also comprised several isoelectric forms.
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Francoeur et al. (1985) studied this question.
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