LIPG SNPs, their haplotypes and gene-environment interactions on serum lipid levels

Q: What is the clinical evidence from this study?

Study design: Cross-Sectional. Population: Healthy subjects (n=2498). Intervention: LIPG single nucleotide polymorphisms (rs3813082, rs2000813, rs2097055, rs3819166) and haplotypes vs. Non-carriers or alternate genotypes. Primary outcome: Serum lipid levels (HDL-C, TC, LDL-C, TG, ApoA1, ApoB) (p=<0.0125).

Shuo Yang; Rui‐Xing Yin; Liu Miao; Yong-Gang Zhou; Jie Wu; Qinghui Zhang

doi:10.1186/s12944-018-0942-y

January 8, 2019Lipids in Health and DiseaseOpen Access

LIPG SNPs, their haplotypes and gene-environment interactions on serum lipid levels

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Key result

LIPG single nucleotide polymorphisms (rs3813082, rs2000813, and rs2097055) and their haplotypes were significantly associated with serum high-density lipoprotein cholesterol levels in both Maonan and Han populations.

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Why the study?

Little is known about the association of endothelial lipase gene single nucleotide polymorphisms and serum lipid levels in Chinese populations.

Do LIPG single nucleotide polymorphisms and their haplotypes affect serum lipid levels in the Maonan and Han populations?

Key Points

Investigate the association of LIPG SNPs and haplotypes with serum lipid levels in two Chinese ethnic groups.
Analyzed 1280 Maonan and 1218 Han participants' serum lipid levels and LIPG SNPs.
Determined genotypes using polymerase chain reaction-restriction fragment length polymorphism and verified with sequencing.
Compared haplotype frequencies and their associations with dyslipidemia between the two ethnic groups.
High-density lipoprotein cholesterol was associated with SNPs rs3813082, rs2000813, and rs2097055 in both groups (P < 0.0125).
Haplotype frequencies differed significantly between Maonan and Han populations, with P-values ranging from < 0.05 to 0.001.
Some haplotypes showed different associations with dyslipidemia across the populations (P < 0.05-0.001).

Study Design

Type

Cross-Sectional (n=2,498)

Multicenter

No

Structured PICO

Do LIPG single nucleotide polymorphisms and their haplotypes affect serum lipid levels in the Maonan and Han populations?

P

Population

2,498 healthy adults aged 25 to 80 years from the Maonan and Han populations in China, free of cardiovascular disease and lipid-lowering medications.

E

Exposure

Genotyping for four endothelial lipase gene (LIPG) single nucleotide polymorphisms (rs3813082, rs2000813, rs2097055, rs3819166)

O

Outcome

Association of LIPG SNPs and their haplotypes with serum lipid levels (TC, TG, HDL-C, LDL-C, ApoA1, ApoB)surrogate

Main Result

p-value: p=<0.0125

Limitations

Sample size was relatively small compared to many GWASes and replication studies.
Not able to alleviate the effect of diet and several environmental factors during the statistical analysis.
Association of LIPG SNPs and serum lipid levels still needs to be verified in different ethnic groups.
Gene expression in adipose tissue was not detected.

Implication

Genetic variations in the LIPG gene and their haplotypes are significantly associated with serum lipid profiles, with differences observed between the Maonan and Han populations in China.

Abstract

BACKGROUND: Maonan nationality is a relatively conservative and isolated minority in the Southwest of China. Little is known about the association of endothelial lipase gene (LIPG) single nucleotide polymorphisms (SNPs) and serum lipid levels in the Chinese populations. METHODS: A total of 1280 subjects of Maonan nationality and 1218 participants of Han nationality were randomly selected from our previous stratified randomized samples. Genotypes of the four LIPG SNPs were determined by polymerase chain reaction-restriction fragment length polymorphism, and then confirmed by direct sequencing. RESULTS: Several SNPs were associated with high-density lipoprotein cholesterol (rs3813082, rs2000813 and rs2097055) in the both ethnic groups; total cholesterol and apolipoprotein (Apo) A1 (rs2000813) in Han nationality; and low-density lipoprotein cholesterol, ApoB, triglyceride (rs2097055) and ApoA1 (rs3819166) in Maonan minority (P < 0.0125 for all after Bonferroni correction). The commonest haplotype was rs3813082T-rs2000813C-rs2097055T-rs3819166A (Han, 44.2% and Maonan, 48.7%). The frequencies of the T-C-T-A, T-C-T-G, T-T-C-G and G-T-C-G haplotypes were different between the Maonan and Han populations (P < 0.05-0.001). The associations between haplotypes and dyslipidemia were also different in the Han and/or Maonan populations (P < 0.05-0.001). CONCLUSIONS: The differences in serum lipid profiles between the two ethnic groups might partly be attributed to these LIPG SNPs, their haplotypes and gene-environmental interactions. TRIAL REGISTRATION: Retrospectively registered.

Discussion

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Cite This Study

Yang et al. (2019) conducted a cross-sectional in Healthy subjects (n=2,498). LIPG single nucleotide polymorphisms (rs3813082, rs2000813, rs2097055, rs3819166) and haplotypes vs. Non-carriers or alternate genotypes was evaluated on Serum lipid levels (HDL-C, TC, LDL-C, TG, ApoA1, ApoB) (p=<0.0125). LIPG single nucleotide polymorphisms (rs3813082, rs2000813, and rs2097055) and their haplotypes were significantly associated with serum high-density lipoprotein cholesterol levels in both Maonan and Han populations.

synapsesocial.com/papers/6a20e674dc4e16663149d62f https://doi.org/10.1186/s12944-018-0942-y

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LIPG SNPs, their haplotypes and gene-environment interactions on serum lipid levels

Figures

Key Points

Study Design

Structured PICO

Main Result

Limitations

Implication

Abstract

Discussion

Cite This Study

LIPG SNPs, their haplotypes and gene-environment interactions on serum lipid levels

Figures

Key Points

Study Design

Structured PICO

Main Result

Limitations

Implication

Abstract

Discussion

Cite This Study

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