Key result
CAD is linked to higher epicardial adipose ceramide biosynthesis gene expression, particularly SPTLC1, vs VHD.
Why the study?
Does coronary artery disease alter the expression of ceramide metabolism enzymes in cardiac adipose tissues compared to valvular heart disease?
Cross-Sectional (n=60)
No
Does coronary artery disease alter the expression of ceramide metabolism enzymes in cardiac adipose tissues compared to valvular heart disease?
Answers are grounded in this paper and cite their sources.
The elevated expression of ceramide-metabolizing enzymes in the epicardial adipose tissue of CAD patients highlights localized lipid metabolic dysregulation that may contribute to coronary atherogenesis.
Here, we examined the expression of ceramide metabolism enzymes in the subcutaneous adipose tissue (SAT), epicardial adipose tissue (EAT) and perivascular adipose tissue (PVAT) of 30 patients with coronary artery disease (CAD) and 30 patients with valvular heart disease (VHD) by means of quantitative polymerase chain reaction and fluorescent Western blotting. The EAT of patients with CAD showed higher expression of the genes responsible for ceramide biosynthesis (SPTLC1, SPTLC2, CERS1, 5, 6, DEGS1, and SMPD1) and utilization (ASAH1, SGMS1). PVAT was characterized by higher mRNA levels of CERS3, CERS4, DEGS1, SMPD1, and ceramide utilization enzyme (SGMS2). In patients with VHD, there was a high CERS4, DEGS1, and SGMS2 expression in the EAT and CERS3 and CERS4 expression in the PVAT. Among patients with CAD, the expression of SPTLC1 in SAT and EAT, SPTLC2 in EAT, CERS2 in all studied AT, CERS4 and CERS5 in EAT, DEGS1 in SAT and EAT, ASAH1 in all studied AT, and SGMS1 in EAT was higher than in those with VHD. Protein levels of ceramide-metabolizing enzymes were consistent with gene expression trends. The obtained results indicate an activation of ceramide synthesis de novo and from sphingomyelin in cardiovascular disease, mainly in EAT, that contributes to the accumulation of ceramides in this location.
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Груздева et al. (2023) conducted a cross-sectional in Coronary artery disease vs. Valvular heart disease (n=60). Coronary artery disease vs. Valvular heart disease was evaluated on Expression of ceramide biosynthesis and utilization genes in epicardial adipose tissue (EAT) (p=0.0022). Patients with coronary artery disease had significantly higher expression of ceramide biosynthesis genes, particularly SPTLC1 (p=0.0022), in epicardial adipose tissue compared to patients with valvular heart disease.
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