What question did this study set out to answer?

The research investigates how nitric oxide influences tumor necrosis factor-alpha expression in the heart.

September 12, 2000Circulation

Nitric Oxide Provokes Tumor Necrosis Factor-α Expression in Adult Feline Myocardium Through a cGMP-Dependent Pathway

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Population

Buffer-perfused Langendorff adult cat hearts and isolated cardiac myocytes

Comparison

NO donor S-nitroso-N-acetyl penicillamine… vs Untreated controls, NO quenching agent, cGMP…

Design

Preclinical

Authors

DKDinesh KalraCardiac Imaging GBGeorg BaumgartenHeart Failure & Transplant ZDZiad DibbsMerck & Co., Inc., Rahway, NJ, USA (United States)

Discussion

Member takes

Implication

NO-driven myocardial TNF-α induction is hypothesis-generating in feline models; leaves open its contribution to human HF inflammation.

Key Points

The research investigates how nitric oxide influences tumor necrosis factor-alpha expression in the heart.
Examined effects of NO donor SNAP in buffer-perfused Langendorff hearts.
Used electrophoretic mobility shift assays to analyze NF-kappaB activation.
Evaluated cGMP pathway involvement with specific antagonists.
SNAP increased TNF-alpha mRNA and protein in a time- and dose-dependent manner.
Effects of SNAP were blocked by NO quenching agent C-PTIO and mirrored by sodium nitroprusside.
8-bromo-cGMP reproduced SNAP effects, showing cGMP pathway's critical role.

Structured PICO

Population

Buffer-perfused Langendorff adult cat hearts and isolated cardiac myocytes

Intervention

NO donor S-nitroso-N-acetyl penicillamine (SNAP) (1 micromol/L), sodium nitroprusside, and 8-bromo-cGMP

Comparator

Untreated controls, NO quenching agent (C-PTIO), cGMP antagonist (ODQ), or protein kinase G antagonist (Rp-8-Br-cGMPS)

Outcome

Myocardial TNF-alpha mRNA and protein biosynthesissurrogate

Nitric oxide provokes TNF-alpha biosynthesis through a cGMP-dependent pathway in adult feline myocardium, suggesting a potential mechanism for persistent inflammation in the failing heart.

Cite This Study

Kalra et al. (2000) studied this question.

synapsesocial.com/papers/6a2116429c438a1c67967dff https://doi.org/10.1161/01.cir.102.11.1302

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Nitric Oxide Provokes Tumor Necrosis Factor-α Expression in Adult Feline Myocardium Through a cGMP-Dependent Pathway

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Nitric Oxide Provokes Tumor Necrosis Factor-α Expression in Adult Feline Myocardium Through a cGMP-Dependent Pathway

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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