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Rapid assessment demonstrates the effectiveness of human interferon in reducing cytopathic effects in vitro, suggesting a quick evaluation method.
A rapid, cytopathic effect reduction assay for human interferon (IFN) is described. Dilutions of IFN were made with an automated diluter in 96-well microtiter plates. Total incubation time was 26 h. IFN titers were calculated from optical density readings of crystal violet-stained monolayers in an automated spectrophotometer, which required less than 1 min to read each plate.
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Yeh et al. (1982) studied this question.
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