Key result
The cytopathic effect inhibition method in microplates was the most sensitive assay for human interferon, while the plaque reduction method was the most reproducible.
Population
Vero cells
Comparison
Assay of human interferon by four methods vs Comparison among the four assay methods
Design
Preclinical
Authors
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May guide interferon assay selection in research; leaves open clinical validation of microplate sensitivity versus plaque reproducibility.
The plaque reduction method offers the best reproducibility for assaying human interferon in Vero cells, while the microplate method yields the highest titers.
Ferreira et al. (1979) studied this question. Assay methods for human interferon was evaluated on Sensitivity and reproducibility of interferon assays. The cytopathic effect inhibition method in microplates was the most sensitive assay for human interferon, while the plaque reduction method was the most reproducible.
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