In vitro Methods and the Study of Platelet Mechanisms

Vox SanguinisVolume 40, Issue s1 p. 22-35 In vitro Methods and the Study of Platelet Mechanisms Marian A. Packham, Corresponding Author Marian A. Packham Department of Biochemistry, University of Toronto, Ont., CanadaDepartment of Biochemistry, University of Toronto, Toronto, Ont., M5S 1A8 (Canada)Search for more papers by this authorJ. Fraser Mustard, J. Fraser Mustard Department of Pathology, McMaster University, Hamilton, Ont., CanadaSearch for more papers by this author Marian A. Packham, Corresponding Author Marian A. Packham Department of Biochemistry, University of Toronto, Ont., CanadaDepartment of Biochemistry, University of Toronto, Toronto, Ont., M5S 1A8 (Canada)Search for more papers by this authorJ. Fraser Mustard, J. Fraser Mustard Department of Pathology, McMaster University, Hamilton, Ont., CanadaSearch for more papers by this author First published: January 1981 https://doi.org/10.1111/j.1423-0410.1981.tb00735.xCitations: 3AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat References 1 Mustard, J. F.; Packham, M. A.: Normal and abnormal haemostasis. Br. med. Bull. 33: 187–193 (1977). 2 Mason, R.G.; Saba, H.I.: Normal and abnormal hemostasis - an integrated view. Am. J. Path. 92: 773–811 (1978). 3 Baumgartner, H.R.; Muggli, R.; Tschopp, T.B.; Turitto, V.T.: Platelet adhesion, release and aggregation in flowing blood: effects of surface properties and platelet function. Thromb. Haemostasis 35: 124–138 (1976). 4 Baumgartner, H. R.; Muggli, R.: Adhesion and aggregation: morphological demonstration and quantitation in vivo and in vitro; in Gordon, Platelets in iology and pathology, pp. 23–60 (Elsevier/North-Holland Biomedical Press, Amsterdam 1976). 5 Cazenave, J.-P.; Packham, M.A.; Guccione, M.A.; Mustard, J. F.: Inhibition of platelet adherence to damaged surface of rabbit aorta. J. Lab. clin. Med. 56: 551–563(1975). 6 Cazenave, J.-P.; Blondowska, D.; Richardson, M.; Kinlough-Rathbone, R. L.; Packham, M.A.; Mustard, J. F.: Quantitative radioisotopic measurement and scanning electron microscopic study of platelet adherence to a collagen-coated surface and to sub-endothelium with a rotating probe device. J. Lab. clin. Med. 93: 60–70 (1979). 7 McGill, M.; Brindley, D.C.: Effects of storage on platelet reactivity to arterial subendothelium during blood flow. J. Lab. clin. Med. 94: 370–380 (1979). 8 Brass, L. F.; Faile, D.; Bensusan, H. B.: Direct measurement of the platelet: collagen interaction by affinity chromatography on collagen/Sepharose. J. Lab. clin. Med. 87: 525–534 (1976). 9 Salzman, E. W.: Measurement of platelet adhesiveness: a simple in vitro technique demonstrating an abnormality in von Willebrand's disease. J. Lab. clin. Med. 62: 724–734 (1963). 10 Bowie, E. J.W.; Owen, C. A., Jr.: Platelet retention and other adhesion-aggregation phenomena; in Holmsen Day, Zucker, Platelet function testing, pp. 160–173. DHEW Publication No. (NIH) 78–1087 (US Government Printing Office, Washington 1978). 11 Zucker, M.B.; McPherson, J.: Reactions of platelets near surfaces in vitro: lessons from the platelet retention test; in Vroman, Leonard, The behaviour of blood and its components at interfaces. Ann. N.Y. Acad. Sci. 283: 128–137 (1977). 12 Tschopp, T.B.; Weiss, H.J.; Baumgartner, H.R.: Decreased adhesion of platelets to subendothelium in von Willebrand's disease. J. Lab. clin. Med. 83: 296–300 (1974). 13 Baumgartner, H.R.; Tschopp, T.B.; Meyer, D.: Shear rate dependent inhibition of platelet adhesion and aggregation on collagenous surfaces by antibodies to human factor VIII/von Willebrand factor. Br. J. Haematol. 44: 127–139 (1980). 14 Flower, R.J.; Blackwell, G.J.: The importance of phospholipase A2 in prostaglandin biosynthesis. Biochem. Pharmac. 25: 285–291 (1976). 15 Marcus, A. J.: The role of lipids in platelet function. J. Lipid Res. 19: 793–826 (1978). 16 Rittenhouse-Simmons, S.: Production of diglyceride from phosphatidyl-inositol in activated human platelets. J. clin. Invest. 63: 580–587 (1979). 17 Bell, R.L.; Kennerly, D.A.; Stanford, N.; Majerus, P.W.: Diglyceride lipase: a pathway for arachidonate release from human platelets. Proc. Natn. Acad. Sci. USA 76: 3238–3241 (1979). 18 Samuelsson, B.: The role of prostaglandin endoper-oxides and thromboxanes in human platelets; in Silver, Smith, Kocsis, Prostaglandins in hematology, pp. 1–10 (Spectrum Publications, New York 1977). 19 Burch, J.W.; Majerus, P.W.: The role of prostaglandins in platelet function. Semin. Hematol. 16: 196–207 (1979). 20 Moncada, S.; Vane, J. R.: Pharmacology and endogenous roles of prostaglandin endoperoxides, thromboxane A2, and prostacyclin. Pharmac. Rev. 30: 293–331 (1978). 21 Lewy, R.L.; Smith, J.B.; Silver, M.J.; Saia, J.; Walinsky, P.; Wiener, L.: Detection of thromboxane B2 in peripheral blood of patients with Prinz-metal's angina. Prostagland. Med. 2: 243–248 (1979). 22 Passwell, J. H.; Dayer, J.-M.; Merler, E.: Increased prostaglandin production by human monocytes after membrane receptor activation. J. Immun. 123: 115–120 (1979). 23 Levine, L.; Cemosek, R.M.G.; Vunakis, H. Van: Specificities of prostaglandins B1, F1α, and F2α, antigen-antibody reactions. J. biol. Chem. 246: 6782–6785 (1971). 24 Okuma, M.; Steiner, M.; Baldini, M.: Studies on lipid peroxides in platelets. II. Effect of aggregating agents and platelet antibody. J. Lab. clin. Med. 77: 728–742 (1971). 25 Smith, J.B.; Ingerman, C.M.; Silver, M.J.: Malon-dialdehyde formation as an indicator of prostaglandin production by human platelets. J. Lab. clin. Med. 88: 167–172 (1976). 26 Stuart, M.J.; Murphy, S.; Oski, F.A.: Simple non-radioisotope technic for the determination of platelet life-span. New Engl. J. Med. 292: 1310–1313 (1975). 27 Marcus, A.J.; Bressler, N.M.; Broekman, M.J.: Oxygen-derived metabolic processes in platelets and leukocytes. Adv. Inflam. Res. 1: 175–181 (1979). 28 Holmsen, H.: Platelet secretion (release reaction); in Mielke, Mechanisms of hemostasis and thrombosis, pp. 73–111 (Symposia Specialists, Miami 1978). 29 Fukami, M.H.; Salganicoff, L.: Human platelet storage organelles A review. Thromb. Haemostasis 38: 963–970(1977). 30 Kinlough-Rathbone, R. L.; Cazenave, J.-P.; Packham, M.A.; Mustard, J.F.: Effect of inhibitors of the arachidonate pathway on the release of granule contents from rabbit platelets adherent to collagen. Lab. Invest. 42: 28–34 (1980). 31 Mills, D. C. B.; Robb, L. A.; Roberts, G. C. K.: The release of nucleotides, 5-hydroxytryptamine and enzymes from human blood platelets during aggregation. J. Physiol. 195: 715–729 (1968). 32 Feinstein, M. B.; Fraser, C.: Human platelet secretion and aggregation induced by calcium iono-phores. Inhibition by PGE1 and dibutyrylcyclic AMP. J. gen. Physiol. 66: 561–581 (1975). 33 Holmsen, H.; Østvold, A.-C.; Day, H. J.: Behaviour of endogenous and newly absorbed serotonin in the platelet release reaction. Biochem. Pharmac. 22: 2599–2608 (1973). 34 Pletscher, A.: Metabolism, transfer and storage of 5-hydroxytryptamine in blood platelets. Br. J. Pharmac. Chemother. 32: 1–16 (1968). 35 Reimers, H.-J.; Allen, D. J.; Cazenave, J.-P.; Feuer-stein, L.A.; Mustard, J.F.: Serotonin transport and storage in rabbit blood platelets - the effects of reserpine and imipramine. Biochem. Pharmac. 26: 1645–1655 (1977). 36 Holmsen, H.; Storm, E.; Day, H. J.: Determination of ATP and ADP in blood platelets: a modification of the firefly luciferase assay for plasma. Analyt. Biochem. 46: 489–501 (1972). 37 Charo, I. F.; Feinman, R. D.; Detwiler, T. C.: Interrelation of platelet aggregation and secretion. J. clin. Invest. 60: 866–873 (1977). 38 Akkerman, J. W. N.; Holmsen, H.; Loughnane, M.: Simultaneous measurement of aggregation, secretion, oxygen uptake, proton production, and intra-cellular metabolites in the same platelet suspension. Analyt. Biochem. 97: 387–393 (1979). 39 Kaplan, K. L.; Nossel, H. L.; Drillings, M.; Lesznik, G.: Radioimmunoassay of platelet factor 4 and β-thromboglobulin: development and application to studies of platelet release in relation to fibrinopeptide A generation. Br. J. Haemat. 39: 129–146 (1978). 40 Rucinski, B.; Niewiarowski, S.; James, P.; Walz, D.A.; Budzynski, A.Z.: Antiheparin proteins secreted by human platelets. Purification, characterization and radioimmunoassay. Blood 53: 47–62 (1979). 41 Bolton, A.E.; Ludlam, C.A.; Moore, S.; Pepper, D. S.; Cash, J. D.: Three approaches to the radioimmunoassay of human β-thromboglobulin. Br, J. Haemat. 33: 233–238 (1976). 42 Handin, R.I.; McDonough, M.; Lesch, M.: Elevation of platelet factor four in acute myocardial infarction: measurement by radioimmunoassay. J. Lab. clin. Med. 91: 340–349 (1978). 43 Chesterman, C.N.; McGready, J.R.; Doyle, D.J.; Morgan, F.J.: Plasma levels of platelet factor 4 measured by radioimmunoassay. Br. J. Haemat. 40: 489–500 (1978). 44 Kinlough-Rathbone, R.L.; Packham, M.A.; Reimers, H.-J.; Cazenave, J.-P.; Mustard, J.F.: Mechanisms of platelet shape change, aggregation, and release induced by collagen, thrombin, or A23187. J. Lab. clin. Med. 90: 707–719 (1977). 45 Packham, M.A.; Kinlough-Rathbone, R.L.; Reimers, H.-J.; Scott, S.; Mustard, J.F.: Mechanisms of platelet aggregation independent of adenosine diphosphate; in Smith Silver, Kocsis, Prostaglandins in hematology, pp. 247–276 (Spectrum Publications, New York 1977). 46 Mustard, J.F.; Packham, M.A.; Kinlough-Rathbone, R. L.; Perry, D.W.; Regoeczi, E.: Fibrinogen and ADP-induced platelet aggregation. Blood 52: 453–466 (1978). 47 Bennett, J.S.; Vilaire, G.: Exposure of human platelet fibrinogen receptors by ADP and epinephrine. J. clin. Invest. 64: 1393–1401 (1979). 48 Marguerie, G.A.; Plow, E. F.; Edgington, T.S.: Human platelets possess an inducible and saturable receptor for fibrinogen. J. biol. Chem. 254: 5357–5363 (1979). 49 Peerschke, E. I.; Zucker, M. B.; Grant, R. A.; Egan, J.J.; Johnson, M.M.: Correlation between fibrinogen binding to human platelets and platelet aggregability. Blood 55: 841–847 (1980). 50 Harfenist, E.J.; Packham, M.A.; Mustard, J.F.: Reversibility of the association of fibrinogen with rabbit platelets exposed to ADP. Blood 56: 189–198 (1980). 51 Tollefsen, D.M.; Majerus, P.W.: Inhibition of human platelet aggregation by monovalent antifibrin-ogen antibody fragments. J. clin. Invest. 55: 1259–1268 (1975). 52 Reimers, H.-J.; Kinlough-Rathbone, R. L.; Cazenave, J.-P.; Senyi, A.F.; Hirsh, J.; Packham, M.A.; Mustard, J. F.: In vitro and in vivo functions of thrombin-treated platelets. Thromb. Haemostasis 35: 151–166 (1976). 53 Kinlough-Rathbone, R.L.; Reimers, H.J.; Mustard, J. F.; Packham, M.A.: Sodium arachidonate can induce platelet shape change and aggregation which are independent of the release reaction. Science 192: 1011–1012 (1976). 54 Packham, M.A.; Kinlough-Rathbone, R.L.; Mustard, J.F.: Aggregation and agglutination; in Day, Holmsen, Zucker, Platelet function testing, pp. 66–92 DHEW Publication No. (NIH) 78–1087 (US Government Printing Office, Washington 1978). 55 Packham, M.A.: Methods for detection of hypersensitive platelets. Thromb. Haemostasis 40: 175–195 (1978). 56 Packham, M.A.; Warrior, E.S.; Glynn, M.F.; Senyi, A.S.; Mustard, J. F.: Alteration of the response of platelets to surface stimuli by pyrazole compounds. J. exp. Med. 126: 171–188 (1967). 57 Evans, G.; Packham, M.A.; Nishizawa, E. E.; Mustard, J.F.; Murphy, E.A.: The effect of acetylsali-cylic acid on platelet function. J. exp. Med. 128: 877–894 (1968). 58 Mustard, J.F.; Perry, D.W.; Kinlough-Rathbone, R.L.; Packham, M.A.: Factors responsible for ADP-induced release reaction of human platelets. Am. J. Physiol. 228: 1757–1765 (1975). 59 Guccione, M. A.; Packham, M. A.; Kinlough-Rathbone, R. L.; Perry, D.W.; Mustard, J. F.: Reactions of polylysine with human platelets in plasma and in suspensions of washed platelets. Thromb. Haemostasis 36: 360–375(1976). 60 Macfarlane, D.E.; Walsh, P.N.; Mills, D.C.B.; Holmsen, H.; Day, H.J.: The role of thrombin in ADP-induced platelet aggregation and release. A critical evaluation. Br. J. Haemat. 30: 457–463 (1975). 61 Zucker, M. B.: Effect of heparin on platelet function. Thromb. Diath. haemorrh. 33: 63–65 (1975). 62 O'Brien, J.R.; Shoobridge, S.M.; Finch, W.J.: Comparison of the effect of heparin and citrate on platelet aggregation. J. clin. Path. 22: 28–31 (1969). 63 Skoza, L.; Zucker, M. B.; Jerushalmy, Z.; Grant, R.: Kinetic studies of platelet aggregation induced by adenosine diphosphate and its inhibition by chelating agents, guanidino compounds, and adenosine. Thromb. Diath. haemorrh. 18: 713–725 (1967). 64 Eika, C.: The platelet aggregating effect of eight commercial heparins. Scand. J. Haematol. 9: 480–482 (1972). 65 Salzman, E.W.; Rosenberg, R.D.; Smith, M.H.; Lindon, J. N.; Favreau, L.: Effect of heparin and heparin fractions on platelet aggregation. J. clin. Invest. 65: 64–73 (1980). 66 Ts'ao, C.-H.; Lo, R.; Raymond, J.: Critical importance of citrate-blood ratio in platelet aggregation studies. Am. J. clin. Path. 65: 518–522 (1976). 67 Hardisty, R.M.; Hutton, R.A.; Montgomery, D.; Rickard, S.; Trebilcock, H.: Secondary platelet aggregation: a quantitative study. Br. J. Haemat. 19: 307–319 (1970). 68 Roper, P.; Drewinko, B.; Hasler, D.; Johnston, D.; Hester, J.; Freireich, E.J.: Effects of time, platelet concentration, and sex on the human platelet aggregation response. Am. J. clin. Path. 71: 263–268 (1979). 69 Mustard, J. F.; Packham, M.A.: Factors influencing platelet function: adhesion, release and aggregation. Pharmacol. Rev. 22: 97–187 (1970). 70 Coller, B. S.; Franza, B. R.; Gralnick, H. R.: The pH dependence of quantitative ristocetin-induced platelet aggregation: theoretical and practical implications. A new device for maintenance of platelet-rich plasma pH. Blood 47: 841–854 (1976). 71 Breddin, K.; Grun, H.; Krzywanek, H. Y.; Schremmer, W.P.: On the measurement of spontaneous platelet aggregation. The platelet aggregation test. III. Methods and first clinical results. Thromb. Haemostasis 35: 669–691 (1976). 72 Rogers, A. B.: The effect of pH on human platelet aggregation induced by epinephrine and ADP. Proc. Soc. exp. Biol. Med. 139: 1100–1103 (1972). 73 Zucker, M.B.: Tests of platelet adhesion, aggregation and release. Thromb. Diath. haemorrh., suppl. 42, pp. 1–12 (1970). 74 Han, P.; Ardlie, N.G.: The influence of pH, temperature, and calcium on platelet aggregation; maintenance of environmental pH and platelet function for in vitro studies in plasma. Br. J. Haemat. 26: 373–389 (1974). 75 Warlow, C.; Corina, A.; Ogston, D.; Douglas, A. S.: The relationship between platelet aggregation and time interval after venepuncture. Thromb. Diath. haemorrh. 31: 133–141 (1974). 76 Kattlove, H.E.; Alexander, B.; White, F.: The effect of cold on platelets. If. Platelet function after short-term storage at cold temperatures. Blood 40: 688–696 (1972). 77 Rossi, E. C.; Louis, G.: A time-dependent increase in the responsiveness of platelet-rich plasma to epinephrine. J. Lab. clin. Med. 85: 300–306 (1975). 78 Rosenstein, R.; Zacharski, L.R.: Effect of temperature on short-term preservation of platelets for in vitro tests. Haemostasis 7: 359–366 (1978). 79 Born, G.V.R.; Cross, M. J.: The aggregation of blood platelets. J. Physiol. 168: 178–195 (1963). 80 Marcus, A.J.; Zucker, M. B.: in The physiology of blood platelets (Grune & Stratton, New York 1965). 81 Coller, B. S.; Gralnick, H. R.: The effect of stir bar size and shape on quantitative platelet aggregation. Thromb. Res. 8: 121–129 (1976). 82 Karpatkin, S.: Human platelet senescence. A. Rev. Med. 23: 101–128 (1972). 83 Corash, L.; Tan, H.; Gralnick, H.R.; Shafer, B.: Heterogeneity of human whole blood platelet sub-populations. I. Relationship between buoyant density, cell volume, and ultrastructure. Blood 49: 71–87 (1977). 84 Holdrinet, A.; Ewals, M.; Reichert, W.J.; Haanen, C.: Mathematical correlation between ADP-in-duced platelet aggregation and subsequent disaggre-gation. A parameter for the velocity of disaggregation. Scand. J. Haematol. 6: 354–364 (1969). 85 Cronberg, S.: Evaluation of platelet aggregation. Coagulation 3: 139–151 (1970). 86 Frojmovic, M. M.: Quantitative parameterization of the light transmission properties of citrated, platelet-rich plasma as a function of platelet and adenosine diphosphate concentrations and temperature. J. Lab. clin. Med. 82: 137–153 (1973). 87 Packham, M.A.; Guccione, M.A.; Greenberg, J.P.; Kinlough-Rathbone, R. L.; Mustard, J. F.: Release of 14C-serotonin during initial platelet changes induced by thrombin, collagen, or A23187. Blood 50: 915–926 (1977). 88 Packham, M.A.; Guccione, M.A.; Chang, P.-L.; Mustard, J. F.: Platelet aggregation and release: effects of low concentrations of thrombin or collagen. Am. J. Physiol. 225: 38–47 (1973). 89 Niewiarowski, S.; Stuart, R.K.; Thomas, D.P.: Activation of intravascular coagulation by collagen. Proc. Soc. exp. Biol. Med. 123: 196–200 (1966). 90 Nemerson, Y.; Pitlick, F. A.: The tissue factor pathway of blood coagulation; in Spaet, Progress in hemostasis and thrombosis, vol. 1, pp. 1–37 (Grune & Stratton, New York 1972). 91 Walsh, P.N.: The effects of collagen and kaolin on the intrinsic coagulant activity of platelets. Evidence for an alternative pathway in intrinsic coagulation not requiring factor XII. Br. J. Haemat. 22: 393–405 (1972). 92 Walsh, P. N.: The role of platelets in the contact phase of blood coagulation. Br. J. Haemat. 22: 237–254 (1972). 93 Østerud, B.; Harper, E.; Rapaport, S.I.; Levine, K. K.: Evidence against collagen activation of platelet-associated factor XI as a mechanism for initiating intrinsic clotting. Scand. J. Haematol. 22: 205–213 (1979). 94 Vecchione, J.; Zucker, M. B.: Procoagulant activity of platelets in recalcified plasma. Br. J. Haemat. 31: 423–428 (1975). 95 Vicic, W.J.; Ratnoff, O.D.; Saito, H.; Goldsmith, G. H.: Platelets and surface-mediated clotting activitv. Br. J. Haemat. 43: 91–98 (1979). 96 Hardisty, R. M.; Hutton, R. A.: Platelet aggregation and the availability of platelet factor 3. Br. J. Haemat. 12: 164–776 (1966). 97 Joist, J.H.; Dolezel, G.; Lloyd, J.V.; Kinlough-Rathbone, R.L.; Mustard, J.F.: Platelet factor 3 availability and the platelet release reaction. J. Lab. din. Med. 84: 474–482 (1974). 98 Zwaal, R.F.A.: Membrane and lipid involvement in blood coagulation. Biochim. biophys. Acta 515: 163–205 (1978). 99 Jackson, C.M.; Nemerson, Y.: Blood coagulation. A. Rev. Biochem. 49: 765–811 (1980). 100 Miletich, J.P.; Jackson, C.M.; Majerus, P.W.: Properties of the factor Xa binding site on human platelets. J. biol. Chem. 253: 6908–6916 (1978). 101 Shuman, M.A.; Tollefsen, D. M.; Majerus, P.W.: The binding of human and bovine thrombin to human platelets. Blood 47: 43–54 (1976). 102 Niewiarowski, S.; Regoeczi, E.; Stewart, G.J.; Senyi, A.; Mustard, J. F.: Platelet interaction with polymerizing fibrin. J. clin. Invest. 51: 685–700 (1972). 103 Mustard, J.F.; Perry, D.W.; Ardlie, N.G.; Pack-ham, M. A.: Preparation of suspensions of washed platelets from humans. Br. J. Haemat. 22: 193–204 (1972). 104 Kinlough-Rathbone, R.L.; Mustard, J.F.; Packham, M.A.; Perry, D.W.; Reimers, H.-J.; Cazenave, J.-P.: Properties of washed human platelets. Thromb. Haemostasis 37: 291–308 (1977). 105 Tangen, O.; Berman, H. J.; Marfey, P.: Gel filtration. A new technique for separation of blood platelets from plasma. Thromb. Diath. haemorrh. 25: 268–278 (1971). 106 Walsh, P. N.: Albumin density gradient separation and washing of platelets and the study of platelet coagulant activities. Br. J. Haemat. 22: 205–217 (1972). 107 Mason, R.G.; Read, M.S.; Shermer, R.W.: Comparison of certain functions of human platelets separated from blood by various means. Am. J. Path. 76: 323–332 (1974). 108 Hutton, R.A.; Howard, M.A.; Deykin, D.; Hardisty, R. M.: Methods for the separation of platelets from plasma. A comparison of functional and morphological integrity. Thromb. Diath. haemorrh. 31: 119–132 (1974). 109 Lages, B.; Scrutton, M.C.; Holmsen, H.: Studies on gel-filtered human platelets: isolation and characterization in a medium containing no added Ca2+, Mg2+ or K+. J. Lab. clin. Med. 85: 811–825 (1975). 110 O'Brien, J. R.: A comparison of platelet aggregation produced by seven compounds and a comparison of their inhibitors. J. clin. Path. 17: 275–281 (1964). 111 Niewiarowski, S.; Thomas, D. P.: Platelet aggregation by ADP and thrombin. Nature 212: 1544–1547 (1966). 112 Ardlie, N.G.; Glew, G.; Schwartz, C. J.: Influence of catecholamines on nucleotide induced platelet aggregation. Nature 212: 415–417 (1966). 113 Silver, M.J.; Smith, J.B.; Ingerman, C.; Kocsis, J.J.: Arachidonic acid-induced human platelet aggregation and prostaglandin formation. Prostaglandins 4: 863–875 (1973). 114 Kinlough-Rathbone, R. L.; Packham, M. A.; Mustard, J. F.: Synergism between platelet aggregating the role of the arachidonate Thromb. Res. (1977). N.; N.: by collagen or epinephrine of platelet responsiveness to aggregation. The role of from platelet J. Lab. clin. Med. (1977). Hirsh, J.; Mustard, J.F.: in washed human platelets to comparison with platelets in plasma. Br. J. Haemat. 25: (1973). Tangen, O.; from platelets in by and other and relation to ADP-induced platelet aggregation. Scand. J. Haematol. (1973). Ardlie, G.; Perry, D.W.; Packham, M.A.; Mustard, J.F.: Influence of on of suspensions of washed rabbit platelets. Proc. Soc. exp. Biol. Med. (1971). S.; F.; P.; J.: Aggregation of human gel-filtered platelets: of and clin. biol. 17: (1972). Holmsen, I.; Holmsen, H.: and characterization of an ADP from human plasma. Thromb. Diath. 26: R. J.: Mechanisms of blood platelet aggregation; in Johnson, of and thrombosis, pp. S.; Holmsen, H.: ADP-induced of platelets in I. studies on aggregation in platelet-rich plasma. Scand. J. Haemat. (1975). J.; L. A.: Preparation and storage of platelet II. influencing platelet and function. Br. J. Haemat. (1976). J.: in platelet A. Rev. Med. 31: (1980). Kaplan, Nossel, Lages, B. A.; Weiss, H. J.; D. S.: Studies of the release from human platelets of the factor for human arterial Res. 42: (1978). Kinlough-Rathbone, R.L.; A.; Packham, M.A.; Reimers, H.-J.; Mustard, J.F.: Effect of on washed rabbit platelets. Thromb. Res. 7: (1975). Reimers, H.J.; Packham, M.A.; Kinlough-Rathbone, R. L.; Mustard, J. F.: Effect of of rabbit platelets with low concentrations of thrombin on their and Br. J. Haemat. 25: (1973). Packham, M.A.; Guccione, M.A.; Cazenave, J.-P.; Kinlough-Rathbone, R. L.; Mustard, J. F.: Effect of ADP on rabbit platelet adherence to surfaces and Proc. 38: (1979). Greenberg, J.; Packham, M.A.; Cazenave, J.-P.; Reimers, H.-J.; Mustard, J.F.: Effects on platelet function of of platelet acid by Lab. Invest. 32: (1975). Greenberg, J.P.; Packham, M.A.; Guccione, M. A.; M. L.; Reimers, H.-J.; Mustard, J. F.: of rabbit platelets in vitro with or Blood 53: (1979). Greenberg, J.P.; Packham, M.A.; R.L.; Mustard, J.F.: aggregation of platelets. Res. (1976). Kinlough-Rathbone, R.L.; Packham, M.A.; Perry, D.W.; Guccione, M. A.; Greenberg, J. P.; Mustard, J. F.: Effect of rabbit on rabbit platelets. Proc. 38: (1979). Morgan, R. K.: Platelet behaviour and in the in The blood platelet in pp. New York 1978). White, R. L.: The effect of storage on platelet membrane and response to thrombin. (1979). Platelet membrane during storage of human platelet Thromb. Res. 8: (1976). 22-35