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October 1, 1979BloodOpen Access

The effect of pretreatment of human or rabbit platelets with chymotrypsin on their responses to human fibrinogen and aggregating agents

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Authors

JGJ. GreenbergMPMA PackhamMGMA Guccione

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Overview

Randomized trial demonstrates altered platelet aggregation responses to fibrinogen in human and rabbit platelets treated with chymotrypsin, suggesting new mechanisms for aggregation.

Key Points

  • This research aims to investigate how chymotrypsin treatment affects platelet responses to fibrinogen and aggregation agents.
  • Washed human and rabbit platelets were pretreated with α-chymotrypsin at concentrations of 1-50 U/ml.
  • Platelet aggregation responses were assessed with human fibrinogen, ADP, thrombin, and collagen, post-treatment.
  • Interactions between chymotrypsin-treated platelets and 125I-fibrinogen were measured during aggregation.
  • Chymotrypsin-treated platelets were less responsive to ADP, thrombin, and collagen, but showed aggregation with fibrinogen.
  • Increased 125I-fibrinogen association was observed in chymotrypsin-treated platelets with ADP, as compared to untreated platelets.
  • The treatment caused rearrangement of membrane components, suggesting exposure of fibrinogen-binding sites.

Cite This Study

Greenberg et al. (1979) studied this question.

synapsesocial.com/papers/6a215912baff55cd040300ebhttps://doi.org/10.1182/blood.v54.4.753.753
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