What question did this study set out to answer?

This study investigates how 2,3-butane-dione monoxime and cytochalasin-D affect heart excitability and contraction.

February 19, 2004ENLIGHTEN (Jurnal Bimbingan dan Konseling Islam)Open Access

The electrophysiological and mechanical effects of 2,3‐butane‐dione monoxime and cytochalasin‐D in the Langendorff perfused rabbit heart

Q: What is the clinical evidence from this study?

Study design: Other. Population: Isolated rabbit hearts (n=16). Intervention: 2,3-butane-dione monoxime (BDM) and cytochalasin-D (Cyto-D) vs. Control. Primary outcome: Left ventricular developed pressure (LVDP), conduction delay (CD), and MAPD90.

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Key result

Both BDM and Cyto-D reduced contractility and had pronounced effects on electrical restitution in isolated rabbit hearts.

Population

Isolated rabbit hearts (Langendorff perfused)

Comparison

2,3-butane-dione monoxime (BDM; 1-20 mmol l) and… vs Control values

Design

Preclinical

Authors

SKSarah KettlewellUniversity of Glasgow NWNicola L. WalkerKing's College London SCStuart M. CobbeElectrophysiology

Discussion

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Implication

Researchers should weigh electrical restitution effects when using BDM or Cyto-D for optical mapping; leaves open optimal uncoupler selection in isolated hearts.

Key Points

This study investigates how 2,3-butane-dione monoxime and cytochalasin-D affect heart excitability and contraction.
Monophasic action potentials were recorded from isolated rabbit hearts.
The study examined varying extracellular calcium levels alongside BDM and Cyto-D.
Conduction delay and action potential duration were measured using standardized protocols.
Left ventricular developed pressure decreased significantly in all conditions (20 mmol l(-1) BDM: 4.5% of control).
Conduction delay increased with both BDM and Cyto-D treatments at specific concentrations.
While both BDM and Cyto-D changed electrical restitution, they did not markedly affect the maximum action potential duration.

Structured PICO

Population

Isolated rabbit hearts used to study the electrophysiological and mechanical effects of BDM and Cyto-D.

Intervention

2,3-butane-dione monoxime (BDM; 1-20 mmol l(-1)) and cytochalasin-D (Cyto-D; 1-5 micromol l(-1)) under varying extracellular [Ca2+] (0.7-2.5 mmol l(-1))

Comparator

Control values

Outcome

Electrophysiological and mechanical effects including left ventricular developed pressure (LVDP), conduction delay (CD), time to 90% repolarisation of the monophasic action potential (MAPD90), and electrical restitutionsurrogate

In isolated rabbit hearts, BDM and Cyto-D reduce contractility but have pronounced effects on electrical restitution, which is important to consider when using these agents to facilitate optical mapping.

Cite This Study

Kettlewell et al. (2004) studied Isolated rabbit hearts (n=16). 2,3-butane-dione monoxime (BDM) and cytochalasin-D (Cyto-D) vs. Control was evaluated on Left ventricular developed pressure (LVDP), conduction delay (CD), and MAPD90. Both BDM and Cyto-D reduced contractility and had pronounced effects on electrical restitution in isolated rabbit hearts.

synapsesocial.com/papers/6a21bbc58d200acffec0ac9a https://doi.org/10.1113/expphysiol.2003.026732

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Implication

Researchers should weigh electrical restitution effects when using BDM or Cyto-D for optical mapping; leaves open optimal uncoupler selection in isolated hearts.

The electrophysiological and mechanical effects of 2,3‐butane‐dione monoxime and cytochalasin‐D in the Langendorff perfused rabbit heart

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

The electrophysiological and mechanical effects of 2,3‐butane‐dione monoxime and cytochalasin‐D in the Langendorff perfused rabbit heart

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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