Contractile deactivation and uncoupling of crossbridges. Effects of 2,3-butanedione monoxime on mammalian myocardium.
Population
Mammalian myocardium preparations including ferret papillary muscle, guinea pig cardiac myocytes, and…
Comparison
1 and 3 mM 2,3-butanedione monoxime vs Baseline/control conditions without BDM
Design
Preclinical
Authors
Discussion
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Implication
BDM effects in animal preparations warrant cautious use as a probe; leaves open its specificity for excitation-contraction studies.
Structured PICO
P
Population
Mammalian myocardium preparations including ferret papillary muscle, guinea pig cardiac myocytes, and skinned fiber preparations from guinea pig, bovine, and canine hearts
I
Intervention
1 and 3 mM 2,3-butanedione monoxime (BDM) (up to 10 mM in skinned fiber preparations)
C
Comparator
Baseline/control conditions without BDM
O
Outcome
Intracellular calcium release, peak twitch force, calcium current, maximal calcium-activated force, and maximal shortening velocitysurrogate
BDM uncouples contractile activation from excitation by affecting both calcium availability and the responsiveness of myofilaments to calcium.
Cite This Study
Gwathmey et al. (1991) studied this question.