Key result
Energy depletion with Amytal and CCCP caused ATP to fall from 27.6 to 0.7 nmol/mg protein and increased free calcium, which reversed upon inhibitor removal.
Population
Single adult rat heart myocytes
Comparison
Energy depletion with 3 mM amobarbital and 2… vs Baseline/control aerobic conditions
Design
Preclinical
Follow-up
25 minutes (15 min depletion, 10 min repletion)
Authors
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No direct clinical implications from rat data; leaves open translation of ATP-calcium dynamics to human cardiomyocytes.
In adult rat cardiomyocytes, transient energy depletion and subsequent repletion leads to profound changes in intracellular calcium handling and ATP levels, culminating in spontaneous contractile activity upon recovery.
Li et al. (1989) studied this question. Amobarbital (Amytal) and carbonyl cyanide m-chlorophenylhydrazone (CCCP) vs. Control values (before/after removal) was evaluated on Intracellular free calcium and ATP levels. Energy depletion with Amytal and CCCP caused ATP to fall from 27.6 to 0.7 nmol/mg protein and increased free calcium, which reversed upon inhibitor removal.
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