Key result
Addition of eukaryotic initiation factor 4F to a cell-free extract promoted 5'-proximal initiation relative to 5'-distal initiation in bicistronic mRNAs.
Population
Mouse astrocytoma cell-free extract translating artificial bicistronic mRNAs based on rabbit beta-globin and…
Comparison
Addition of exogenous reticulocyte initiation… vs Absence of added eukaryotic initiation factor 4F
Design
Preclinical
Authors
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Does not inform practice; leaves open eIF4F effects on ORF selection in vivo.
Eukaryotic initiation factor 4F promotes 5'-proximal initiation in bicistronic mRNAs, suggesting its activity levels regulate relative ORF expression.
Tahara et al. (1991) studied this question. Eukaryotic initiation factor 4F vs. Baseline extract (absence of added factor) was evaluated on Translation activity (5'-proximal vs 5'-distal initiation). Addition of eukaryotic initiation factor 4F to a cell-free extract promoted 5'-proximal initiation relative to 5'-distal initiation in bicistronic mRNAs.
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