Key result
SF2/ASF and SC35 stimulate splicing of c-src pre-mRNA, whereas hnRNP A1 and PTB repress exon N1 splicing through different mechanisms in vitro.
The study demonstrates that the exonic splicing regulators SF2/ASF and hnRNP A1 modulate the splicing of c-src exon N1, an exon primarily controlled by intronic factors.
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These in vitro mechanisms should not yet inform src-targeted therapies; leaves open their relevance to exon N1 splicing in disease models.
Rooke et al. (2003) studied this question. SR Proteins (SF2/ASF, SC35) and hnRNP A1 vs. Control (unmutated/unsupplemented in vitro splicing reactions) was evaluated on In vitro splicing of c-src exon N1. SF2/ASF and SC35 stimulate splicing of c-src pre-mRNA, whereas hnRNP A1 and PTB repress exon N1 splicing through different mechanisms in vitro.
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