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ABSTRACT A fluorimetric method for the determination of the free 11-hydroxycorticosteroids (cortisol and corticosterone) in human plasma has been described. Using a routine fluorimeter, 1–2 ml plasma samples are needed for a valid determination. The procedure is reliable as judged by the criteria of Borth (1957). One single trained technician can perform 40–50 determinations a day. Background fluorescence equivalent to about 3 μg % hydrocortisone is found; this background fluorescence does not appear to be related to plasma cholesterol. The method is designed primarily to study the dynamics of adrenocortical function in a given individual and can be of help as an emergency screening test (a determination can be done in one hour) in cases of suspected adrenocortical dysfunction.
Moor et al. (Mon,) studied this question.