Key result
Doxorubicin (8 x 10(-6) M) induced alterations in the distribution of actin fluorescence on the neural tube and CEF cell cytoskeleton, and increased the critical concentration of monomeric actin.
Why the study?
Does doxorubicin alter actin polymerization in early chick embryos and CEF cells?
Does doxorubicin alter actin polymerization in early chick embryos and CEF cells?
Doxorubicin treatment alters actin polymerization and distribution in early chick embryos and CEF cells, suggesting a mechanism for its cellular effects.
Answers are grounded in this paper and cite their sources.
No clinical changes warranted; hypothesis-generating for doxorubicin actin effects in embryonic models.
The inhibition of neural tube closure in early chick embryos explanted in vitro by New's technique was used as a model to study the possible involvement of actin polymerization during doxorubicin treatment. The investigation was performed with indirect immunofluorescence staining, fluorescence staining with NBD-Ph, and DNase I inhibition activity. 8 x 10(-6) M doxorubicin induces alteration in the distribution of actin fluorescence on neural tube and in CEF cell cytoskeleton, and an appreciable increase in the critical concentration of monomeric actin as measured in cell extracts.
No takes yet. Share an insight, caveat, or question.
Necco et al. (1983) studied Neural tube closure. Doxorubicin was evaluated on Distribution of actin fluorescence and critical concentration of monomeric actin. Doxorubicin (8 x 10(-6) M) induced alterations in the distribution of actin fluorescence on the neural tube and CEF cell cytoskeleton, and increased the critical concentration of monomeric actin.
Synapse has enriched 10 related papers. Consider them for comparative context: