Key result
Mice over-expressing both creatine transporter and creatine kinase in the heart exhibited a 51% higher PCr/ATP ratio (3.01 vs 2.04) compared to single transgenic mice.
Why the study?
High heart rate and diminutive size pose challenges for in vivo mouse 31P-CMRS, and whether combined elevation of myocardial creatine and creatine kinase synergistically augments energetic status was unknown.
Does double transgenic expression of Creatine Transporter and Creatine Kinase increase the PCr/ATP ratio in the mouse heart compared to single transgenic expression?
Population
7 CrT-OE and 6 dTg mice
Comparison
dTg mice (CrT-OE and CK) vs CrT-OE mice
Design
Preclinical comparative imaging and hemodynamic study
Authors
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Does not inform clinical practice; leaves open whether dual overexpression could enhance energetics in human heart failure.
Does double transgenic expression of Creatine Transporter and Creatine Kinase increase the PCr/ATP ratio in the mouse heart compared to single transgenic expression?
Mean Difference: 0.97
Absolute Event Rate: 3.01% vs 2.04%
A refined high-resolution 31P-CMRS technique demonstrated that mice over-expressing both creatine and CK in the heart exhibit a synergistic elevation in PCr/ATP, suggesting therapeutic potential for chronic heart failure.
Maguire et al. (2023) studied Myocardial energetics (n=13). Double transgenic (dTg) overexpression of creatine transporter and creatine kinase vs. Single transgenic Creatine Transporter overexpressing (CrT-OE) mice was evaluated on Myocardial PCr/ATP ratio (51% higher). Mice over-expressing both creatine transporter and creatine kinase in the heart exhibited a 51% higher PCr/ATP ratio (3.01 vs 2.04) compared to single transgenic mice.
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