February 1, 1994Journal of Clinical InvestigationOpen Access

Prostaglandin E2 activates clusters of apical Cl- channels in principal cells via a cyclic adenosine monophosphate-dependent pathway.

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Structured PICO

Population

Primary cultured principal cells from cortical collecting tubules of New Zealand white rabbits (1-2 kg)

Intervention

Basolateral exposure to 0.5 microM PGE2, 10 microM forskolin, or 0.5 mM dibutyryl cyclic adenosine monophosphate (cAMP) for 5 to 30 minutes

Comparator

Basal conditions (untreated patches) or exposure to apical PGE2, PGF2 alpha, or thapsigargin

Outcome

Channel activity measured as number of channels times open probability (NP0) and channel frequencysurrogate

Basolateral PGE2 activates clustered apical 9-pS Cl- channels in rabbit cortical collecting tubule principal cells via a cAMP-dependent, Ca2+-independent pathway.

Discussion

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Implication

Supports cAMP-mediated Cl- channel regulation in rabbit principal cells; leaves open relevance to human renal physiology or disease.

Cite This Study

Ling et al. (1994) studied this question.

synapsesocial.com/papers/6a229ab6b277bb7f4c149dbe https://doi.org/10.1172/jci117037

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Prostaglandin E2 activates clusters of apical Cl- channels in principal cells via a cyclic adenosine monophosphate-dependent pathway.

Structured PICO

Discussion

Implication

Cite This Study

Prostaglandin E2 activates clusters of apical Cl- channels in principal cells via a cyclic adenosine monophosphate-dependent pathway.

Structured PICO

Discussion

Implication

Cite This Study

Also Consider

Also Consider