Key result
Amiloride (1 to 10 microM) reduced the steady-state open probability of a Ca2+-dependent, nonselective cation channel in adult rat alveolar type II cells 10-fold without affecting conductance.
Population
Cultured adult rat alveolar type II epithelial cells (held in culture for 24 to 72 h)
Design
Preclinical
Authors
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Does not support clinical use in pulmonary edema; leaves open a role for this channel in alveolar Na+ transport pending in vivo studies.
Demonstrates the presence of an amiloride-sensitive, nonselective cation channel in adult rat alveolar type II cells, which may play a role in Na+ transport and lung liquid clearance.
Feng et al. (1993) studied Pulmonary edema (cellular mechanisms). Amiloride was evaluated on Single-channel currents and channel characteristics. Amiloride (1 to 10 microM) reduced the steady-state open probability of a Ca2+-dependent, nonselective cation channel in adult rat alveolar type II cells 10-fold without affecting conductance.
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