Key result
Cysteine susceptibility analysis and epitope tagging revealed that the connecting loop in the alpha-1 repeat forms a re-entrant membrane loop, suggesting the exchanger has nine transmembrane helices.
Population
Cardiac Na+/Ca2+ exchanger
Design
Preclinical
Authors
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Refines NCX topology model; hypothesis-generating for structure-function studies in heart failure.
This study proposes a revised nine-transmembrane helix topology for the cardiac Na+/Ca2+ exchanger based on cysteine susceptibility and epitope tagging.
Iwamoto et al. (1999) studied this question. Cysteine susceptibility analysis and epitope tagging was evaluated on Membrane topology of the cardiac Na+/Ca2+ exchanger. Cysteine susceptibility analysis and epitope tagging revealed that the connecting loop in the alpha-1 repeat forms a re-entrant membrane loop, suggesting the exchanger has nine transmembrane helices.
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