Key result
Cytoplasmic acidification produced a biphasic but monotonic decrease in sodium-calcium exchange current, reflecting both a rapid primary inhibition and a slow secondary block by protons.
Population
Giant excised membrane patches obtained from cardiac myocytes of the adult guinea-pig
Comparison
Changes in cytoplasmic pH, sodium, calcium, and… vs Baseline conditions or constant cytoplasmic pH
Design
Preclinical
Authors
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May modulate NCX in acidotic myocytes; extends proton-regulation mechanisms but leaves open translation to human ischemia.
Cytoplasmic protons inhibit the cardiac sodium-calcium exchanger via a biphasic mechanism involving a proton-sensitive regulatory component that can be removed by alpha-chymotrypsin.
DOERING et al. (1993) studied this question. Cytoplasmic acidification (increase in [H+]i) was evaluated on Sodium-calcium exchange current (INa-Ca). Cytoplasmic acidification produced a biphasic but monotonic decrease in sodium-calcium exchange current, reflecting both a rapid primary inhibition and a slow secondary block by protons.
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