Key result
At least three Ca2+-sensitive protein kinases, including phosphorylase kinase and protein kinase C, are involved in the response of hepatocytes to Ca2+-linked hormones.
Population
Hepatocytes from gsd/gsd rats (deficient in phosphorylase kinase) and normal Wistar controls
Comparison
Stimulation with agonists including glucagon… vs Normal Wistar control hepatocytes or…
Design
Preclinical
Authors
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Should not yet inform clinical kinase targeting in liver disease; leaves open validation in human models.
This preclinical study demonstrates that hormones like vasopressin and angiotensin II generate multiple intracellular messengers (diacylglycerol and Ca2+) to activate distinct protein kinases in hepatocytes.
Garrison et al. (1984) studied this question. Angiotensin II, vasopressin, phorbol esters, and A23187 vs. Unstimulated cells / normal Wistar controls was evaluated on Phosphorylation state of hepatic cytosolic proteins. At least three Ca2+-sensitive protein kinases, including phosphorylase kinase and protein kinase C, are involved in the response of hepatocytes to Ca2+-linked hormones.
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