February 1, 1989Molecular and Cellular Biology

The Poly(A)-Poly(A)-Binding Protein Complex Is a Major Determinant of mRNA Stability In Vitro

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Population

In vitro mRNA decay system using cell extracts and polyadenylated mRNAs

Comparison

Depletion of functional PABP (via excess poly… vs Reactions containing excess PABP or…

Design

Preclinical

Authors

PBPhilip BernsteinUniversity of Wisconsin–Madison SPStuart W. PeltzRutgers, The State University of New Jersey JRJeffrey RossRoswell Park Comprehensive Cancer Center

Discussion

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Implication

Should not change practice; extends in vitro evidence for PABP in mRNA stability but leaves cardiac translation open.

Structured PICO

Population

In vitro mRNA decay system using cell extracts and polyadenylated mRNAs (beta-globin, chloramphenicol acetyltransferase, and simian virus 40 virion proteins)

Intervention

Depletion of functional PABP (via excess poly(A) competitor or poly(A)-Sepharose column) and addition of purified Saccharomyces cerevisiae or human cytoplasmic PABP

Comparator

Reactions containing excess PABP or nonpolyadenylated mRNAs

Outcome

mRNA stability and decay ratesurrogate

The poly(A)-PABP complex is a major determinant of mRNA stability in vitro, protecting polyadenylated mRNAs from indiscriminate destruction by cellular nucleases.

Cite This Study

Bernstein et al. (1989) studied this question.

synapsesocial.com/papers/6a23c611ffed3004559eef3f https://doi.org/10.1128/mcb.9.2.659-670.1989

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The Poly(A)-Poly(A)-Binding Protein Complex Is a Major Determinant of mRNA Stability In Vitro

Discussion

Member takes

Implication

Structured PICO

Cite This Study

The Poly(A)-Poly(A)-Binding Protein Complex Is a Major Determinant of mRNA Stability In Vitro

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider