Phosphorylation of Smooth Muscle Myosin Heads Regulates the Head-induced Movement of Tropomyosin

It has been shown that skeletal and smooth muscle myosin heads binding to actin results in the movement of smooth muscle tropomyosin, as revealed by a change in fluorescence resonance energy transfer between a fluorescence donor on tropomyosin and on actin tropomyosin movement as a of and smooth muscle myosin the of and myosin heads a change in energy the of the of in that of myosin to energy It that the of tropomyosin on actin by of myosin heads a in the of smooth muscle by myosin heads as by fluorescence resonance energy transfer and between and the binding the binding of and myosin heads to actin and It has been shown that skeletal and smooth muscle myosin heads binding to actin results in the movement of smooth muscle tropomyosin, as revealed by a change in fluorescence resonance energy transfer between a fluorescence donor on tropomyosin and on actin tropomyosin movement as a of and smooth muscle myosin the of and myosin heads a change in energy the of the of in that of myosin to energy It that the of tropomyosin on actin by of myosin heads a in the of smooth muscle by myosin heads as by fluorescence resonance energy transfer and between and the binding the binding of and myosin heads to actin and smooth muscle tropomyosin fluorescence resonance energy transfer of skeletal muscle myosin of smooth muscle myosin a of the actin a of to the of actin and myosin the myosin of energy the movement to the of myosin heads the actin in the and the on by the muscle skeletal binding to the to tropomyosin and actin in the the of the It that the to of of the myosin binding on actin by a that on the actin actin It that the movement of tropomyosin by the binding of to the of in of tropomyosin actin and tropomyosin on the to the binding of myosin heads to the to in the on of skeletal muscle by a of the of tropomyosin between on and the on in and a a movement of of skeletal muscle actin to shown that the movement of skeletal tropomyosin, the myosin binding on actin to that to and that myosin heads to muscle by the of to the of myosin in the and by in of that the myosin by myosin of It the in in the and the smooth muscle actin a smooth muscle of tropomyosin and smooth and It that of smooth muscle tropomyosin to the myosin in of the of and the in myosin in myosin to on smooth muscle the and the of in of of of has been that of of that in the that the movement of skeletal muscle tropomyosin a in the of skeletal muscle the as to a movement of smooth muscle tropomyosin fluorescence resonance energy transfer smooth muscle tropomyosin the binding to actin of and of skeletal muscle myosin and the of smooth muscle myosin in of by in the the movement of smooth muscle tropomyosin by the muscle of smooth muscle myosin by that the movement of tropomyosin by the of the of the movement of tropomyosin in the on and of smooth muscle that the of smooth muscle to the movement of tropomyosin the in in the the in the the in the by actin to and a of the and to the of in a of the of the actin and in a to the of muscle myosin to and of by and to between and as by and of the of smooth muscle by of myosin to and to as to by a myosin smooth muscle myosin as and the that and and as of to of actin as by the change in fluorescence of the donor to of smooth muscle tropomyosin in the of the to actin as a of as in and that to energy as in the of the of actin on the fluorescence of the in fluorescence to the of of and by the in the It of the change in the and the of to the change in fluorescence of in the of the of fluorescence the of on the fluorescence of to by by by to in and in to the the fluorescence of in the of actin fluorescence the of a of on fluorescence and to the the fluorescence of in the of and actin in fluorescence to in to of change in on the fluorescence of on fluorescence to It to the of on the of to actin in the of change in the change the and change and that and on the of a change as the in that the energy transfer on the of the a change in a in the the movement of smooth muscle tropomyosin by myosin heads by the change in between a donor to tropomyosin and a to actin the of fluorescence resonance energy smooth muscle tropomyosin a of a in the of the and a a the the donor to of the in the the donor a change in fluorescence that the of to a in fluorescence to a transfer of energy to on actin transfer to a of between the donor and in the energy transfer between a donor and to the of in the of in in fluorescence to a in between tropomyosin donor and actin of and to a movement of the change in by the smooth muscle myosin change in fluorescence as a of and of that change fluorescence to a of the fluorescence a in and to the the fluorescence to of in the the in change to a in binding to of fluorescence in the of as a of as in the to the of the between and the change in as in the of on of on the binding of to the binding of binding of as the a binding the of of on a of of to and change in of fluorescence in the of as a of in the of as in the to of to the in the of as a of as in in a fluorescence on a a of the in the of the change in fluorescence between and to the between the skeletal muscle myosin and that to and that the myosin the tropomyosin the in the that in the of the movement of by in binding of to actin in the to actin and the binding of a has on the movement of that binding heads and in the of heads of to actin and and as a of to actin the the of tropomyosin the of actin to the the of actin the movement of tropomyosin, by the binding of to the actin of and of of that the change in fluorescence to the of tropomyosin of in that actin the movement of tropomyosin and in actin the movement of tropomyosin in by the that heads of to the and a the the of that a of as to the a between and the of on tropomyosin in between and has been by in the of to on of smooth muscle tropomyosin movement by myosin of to the change in heads as a binding the the the of the of actin the of actin the movement of by the binding of to the actin of fluorescence resonance energy transfer on the of the donor and and to in the to a of a of the donor a the the of of in the the of the the of the in that in myosin heads to as to in between donor and the of that to the of the of the of the on to actin by the fluorescence a to and a of change in to a change of of the results in the the between and on between and of that of the that of the to the movement of tropomyosin as that the of of the in that between and that between the and to in movement of on actin as to the movement of of to in on actin the in that that the between of and of actin to the of heads to a of in the of tropomyosin and to to actin of to the of by myosin heads results that the actin binding of by the binding of myosin heads to actin the of on actin to the of myosin heads on of the on actin that of to actin as revealed by the of of and to actin in the of in the of and in the of of and as of of in and on between and in the of tropomyosin as a of of to in a in fluorescence as a of energy transfer to of transfer in the actin a in of a of in change in fluorescence in and to the the of to a of a in fluorescence to a in binding to to of of the binding of to and the fluorescence to a of between and that binding to actin the results and change in the on the change in between and of and in the of in as of of of that in by to in between donor and and to the movement of tropomyosin on the that the that in the in of on the of to that the binding of to the the of the on on the fluorescence of to actin that the the on the of the of to a on the that change between and between and that in between the and to in of the of a to of that the of the change the binding of myosin heads to actin the that in to in that the in of the the of to in to in between and to a movement of movement of tropomyosin by smooth muscle myosin heads by the of the of in to tropomyosin the of the in that heads to to movement of to to the movement of in in the myosin the movement of tropomyosin by skeletal muscle myosin heads to the of the of to the that to smooth muscle myosin to the skeletal muscle in a change in smooth muscle myosin in skeletal myosin muscle tropomyosin the of smooth muscle myosin by It that to a myosin to the tropomyosin has the on the and the binding of myosin heads to actin the tropomyosin a to a and the of on the in the of the of that the of tropomyosin the to the to the movement of tropomyosin to a on actin that and that the myosin heads in to a a tropomyosin movement in myosin in in smooth muscle in tropomyosin on the the of smooth muscle myosin by in the of the of to of the of in to a has been to a of smooth muscle the that by myosin and the the myosin to and It has been that the of the the of by actin that myosin heads in the of tropomyosin to the the the to tropomyosin to the the of the of in energy by the muscle in change in the of tropomyosin to the muscle the of smooth muscle tropomyosin on actin of between the on and of skeletal muscle tropomyosin smooth muscle tropomyosin that the of the myosin to actin smooth muscle by that myosin in the by that the of tropomyosin on the to to the of between the of myosin and in smooth to the movement of tropomyosin by myosin the binding of myosin heads to actin to a of as revealed by the of change of and between and to results the binding of and the binding of and myosin heads to the actin the binding of myosin heads on of on the of myosin heads to the actin and by the binding to the to the of actin and myosin the myosin of energy the movement to the of myosin heads the actin in the and the on by the muscle skeletal binding to the to tropomyosin and actin in the the of the It that the to of of the myosin binding on actin by a that on the actin actin It that the movement of tropomyosin by the binding of to the of in of tropomyosin actin and tropomyosin on the to the binding of myosin heads to the to in the on of skeletal muscle by a of the of tropomyosin between on and the on in and a a movement of of skeletal muscle actin to shown that the movement of skeletal tropomyosin, the myosin binding on actin to that to and that myosin heads to muscle by the of to the of myosin in the and by in of that the myosin by myosin of It the in in the and the smooth muscle actin a smooth muscle of tropomyosin and smooth and It that of smooth muscle tropomyosin to the myosin in of the of and the in myosin in myosin to on smooth muscle the and the of in of of of has been that of of that in the that the movement of skeletal muscle tropomyosin a in the of skeletal muscle the as to a movement of smooth muscle tropomyosin fluorescence resonance energy transfer smooth muscle tropomyosin the binding to actin of and of skeletal muscle myosin and the of smooth muscle myosin in of by in the the movement of smooth muscle tropomyosin by the muscle of smooth muscle myosin by that the movement of tropomyosin by the of the of the movement of tropomyosin in the on and of smooth muscle that the of smooth muscle to the movement of tropomyosin the in in the the in the the in the by actin to and a of the and to the of in a of the of the actin and in a to the of muscle myosin to and of by and to between and as by and of the of smooth muscle by of myosin to and to as to by a myosin smooth muscle myosin as and the that and and as of to of actin as by the change in fluorescence of the donor to of smooth muscle tropomyosin in the of the to actin as a of as in and that to energy as in the of the of actin on the fluorescence of the in fluorescence to the of of and by the in the It of the change in the and the of to the change in fluorescence of in the of the of fluorescence the of on the fluorescence of to by by by to in and in to the the fluorescence of in the of actin fluorescence the of a of on fluorescence and to the the fluorescence of in the of and actin in fluorescence to in to of change in on the fluorescence of on fluorescence to It to the of on the of to actin in the of change in the change the and change and that and on the of a change as the in that the energy transfer on the of the a change in a the in in the the in the the in the by actin to and a of the and to the of in a of the of the actin and in a to the of muscle myosin to and of by and to between and as by and of the of smooth muscle by of myosin to and to as to by a myosin smooth muscle myosin as and the that and and as of to of actin as by the change in fluorescence of the donor to of smooth muscle tropomyosin in the of the to actin as a of as in and that to energy as in the of the of actin on the fluorescence of the in fluorescence to the of of and by the in the It of the change in the and the of to the change in fluorescence of in the of the of fluorescence the of on the fluorescence of to by by by to in and in to the the fluorescence of in the of actin fluorescence the of a of on fluorescence and to the the fluorescence of in the of and actin in fluorescence to in to of change in on the fluorescence of on fluorescence to It to the of on the of to actin in the of change in the change the and change and that and on the of a change as the in that the energy transfer on the of the a change in a in the the movement of smooth muscle tropomyosin by myosin heads by the change in between a donor to tropomyosin and a to actin the of fluorescence resonance energy smooth muscle tropomyosin a of a in the of the and a a the the donor to of the in the the donor a change in fluorescence that the of to a in fluorescence to a transfer of energy to on actin transfer to a of between the donor and in the energy transfer between a donor and to the of in the of in in fluorescence to a in between tropomyosin donor and actin of and to a movement of the change in by the smooth muscle myosin change in fluorescence as a of and of that change fluorescence to a of the fluorescence a in and to the the fluorescence to of in the the in change to a in binding to the of the between and the change in as in the of on of on the binding of to the binding of binding of as the a binding the of of on a of of to and change in of fluorescence in the of as a of in the of as in the to of to the in the of as a of as in in a fluorescence on a a of the in the of the change in fluorescence between and to the between the skeletal muscle myosin and that to and that the myosin the tropomyosin the in the that in the of the movement of by in binding of to actin in the to actin and the binding of a has on the movement of that binding heads and in the of heads of to actin and and as a of to actin the the of tropomyosin the of actin to the the of actin the movement of tropomyosin, by the binding of to the actin of and of of that the change in fluorescence to the of tropomyosin of in that actin the movement of tropomyosin and in actin the movement of tropomyosin in by the that heads of to the and a the the of that a of as to the a between and the of on tropomyosin in between and has been by in the of to on of smooth muscle tropomyosin movement by myosin of to the change in heads as a binding the the the of the of actin the of actin the movement of by the binding of to the actin of fluorescence resonance energy transfer on the of the donor and and to in the to a of a of the donor a the the of of in the the of the the of the in that in myosin heads to as to in between donor and the of that to the of the of the of the on to actin by the fluorescence a to and a of change in to a change of of the results in the the between and on between and of that of the that of the to the movement of tropomyosin as that the of of the in that between and that between the and to in movement of on actin as to the movement of of to in on actin the in that that the between of and of actin to the of heads to a of in the of tropomyosin and to to actin of to the of by myosin heads results that the actin binding of by the binding of myosin heads to actin the of on actin to the of myosin heads on of the on actin that of to actin as revealed by the of of and to actin in the of in the of and in the of of and as of of in and on between and in the of tropomyosin as a of of to in a in fluorescence as a of energy transfer to of transfer in the actin a in of a of in change in fluorescence in and to the the of to a of a in fluorescence to a in binding to to of of the binding of to and the fluorescence to a of between and that binding to actin the results and change in the on the change in between and of and in the of in as of of of in the the movement of smooth muscle tropomyosin by myosin heads by the change in between a donor to tropomyosin and a to actin the of fluorescence resonance energy smooth muscle tropomyosin a of a in the of the and a a the the donor to of the in the the donor a change in fluorescence that the of to a in fluorescence to a transfer of energy to on actin transfer to a of between the donor and in the energy transfer between a donor and to the of in the of in in fluorescence to a in between tropomyosin donor and actin of and to a movement of the change in by the smooth muscle myosin change in fluorescence as a of and of that change fluorescence to a of the fluorescence a in and to the the fluorescence to of in the the in change to a in binding to the of the between and the change in as in the of on of on the binding of to the binding of binding of as the a binding the of of on a of of to and change in as in fluorescence on a a of the in the of the change in fluorescence between and to the between the skeletal muscle myosin and that to and that the myosin the tropomyosin the in the that in the of the movement of by in binding of to actin in the to actin and the binding of a has on the movement of that binding heads and in the of heads of to actin and and as a of to actin the the of tropomyosin the of actin to the the of actin the movement of tropomyosin, by the binding of to the actin of and of of that the change in fluorescence to the of tropomyosin of in that actin the movement of tropomyosin and in actin the movement of tropomyosin in by the that heads of to the and a the the of that a of as to the a between and the of on tropomyosin in between and has been by in the of to on of fluorescence resonance energy transfer on the of the donor and and to in the to a of a of the donor a the the of of in the the of the the of the in that in myosin heads to as to in between donor and the of that to the of the of the of the on to actin by the fluorescence a to and a of change in to a change of of the results in the the between and on between and of that of the that of the to the movement of tropomyosin as that the of of the in that between and that between the and to in movement of on actin as to the movement of of to in on actin the in that that the between of and of actin to the of heads to a of in the of tropomyosin and to to actin of to the of by myosin heads results that the actin binding of by the binding of myosin heads to actin the of on actin to the of myosin heads on of the on actin that between and in the of tropomyosin as a of of to in a in fluorescence as a of energy transfer to of transfer in the actin a in of a of in change in fluorescence in and to the the of to a of a in fluorescence to a in binding to to of of the binding of to and the fluorescence to a of between and that binding to actin the results and change in the on the change in between and that in by to in between donor and and to the movement of tropomyosin on the that the that in the in of on the of to that the binding of to the the of the on on the fluorescence of to actin that the the on the of the of to a on the that change between and between and that in between the and to in of the of a to of that the of the change the binding of myosin heads to actin the that in to in that the in of the the of to in to in between and to a movement of movement of tropomyosin by smooth muscle myosin heads by the of the of in to tropomyosin the of the in that heads to to movement of to to the movement of in in the myosin the movement of tropomyosin by skeletal muscle myosin heads to the of the of to the that to smooth muscle myosin to the skeletal muscle in a change in smooth muscle myosin in skeletal myosin muscle tropomyosin the of smooth muscle myosin by It that to a myosin to the tropomyosin has the on the and the binding of myosin heads to actin the tropomyosin a to a and the of on the in the of the of that the of tropomyosin the to the to the movement of tropomyosin to a on actin that and that the myosin heads in to a a tropomyosin movement in myosin in in smooth muscle in tropomyosin on the the of smooth muscle myosin by in the of the of to of the of in to a has been to a of smooth muscle the that by myosin and the the myosin to and It has been that the of the the of by actin that myosin heads in the of tropomyosin to the the the to tropomyosin to the the of the of in energy by the muscle in change in the of tropomyosin to the muscle the of smooth muscle tropomyosin on actin of between the on and of skeletal muscle tropomyosin smooth muscle tropomyosin that the of the myosin to actin smooth muscle by that myosin in the by that the of tropomyosin on the to to the of between the of myosin and in smooth to the movement of tropomyosin by myosin the binding of myosin heads to actin to a of as revealed by the of change of and between and to results the binding of and the binding of and myosin heads to the actin the binding of myosin heads on of on the of myosin heads to the actin and by the binding to the that in by to in between donor and and to the movement of tropomyosin on the that the that in the in of on the of to that the binding of to the the of the on on the fluorescence of to actin that the the on the of the of to a on the that change between and between and that in between the and to in of the of a to of that the of the change the binding of myosin heads to actin the that in to in that the in of the the of to in to in between and to a movement of movement of tropomyosin by smooth muscle myosin heads by the of the of in to tropomyosin the of the in that heads to to movement of to to the movement of in in the myosin the movement of tropomyosin by skeletal muscle myosin heads to the of the of to the that to smooth muscle myosin to the skeletal muscle in a change in smooth muscle myosin in skeletal myosin muscle tropomyosin the of smooth muscle myosin by It that to a myosin to the tropomyosin has the on the and the binding of myosin heads to actin the tropomyosin a to a and the of on the in the of the of that the of tropomyosin the to the to the movement of tropomyosin to a on actin that and that the myosin heads in to a a tropomyosin movement in myosin in in smooth muscle in tropomyosin on the the of smooth muscle myosin by in the of the of to of the of in to a has been to a of smooth muscle the that by myosin and the the myosin to and It has been that the of the the of by actin that myosin heads in the of tropomyosin to the the the to tropomyosin to the the of the of in energy by the muscle in change in the of tropomyosin to the muscle the of smooth muscle tropomyosin on actin of between the on and of skeletal muscle tropomyosin smooth muscle tropomyosin that the of the myosin to actin smooth muscle by that myosin in the by that the of tropomyosin on the to to the of between the of myosin and in smooth to the movement of tropomyosin by myosin the binding of myosin heads to actin to a of as revealed by the of change of and between and to results the binding of and the binding of and myosin heads to the actin the binding of myosin heads on of on the of myosin heads to the actin and by the binding to the the and of a of the and and