Assessment of Antibacterial Activity of Crude and Partially Purified Stem-Bark Extracts of Alstonia boonei Against Multidrug-Resistant Bacterial Pathogens
Randomized trial evaluates antibacterial effects of Alstonia boonei extracts on multidrug-resistant bacteria, indicating its potential as a drug source.
Key Points
This study aims to evaluate the antibacterial activity of crude and solvent-fractionated extracts of Alstonia boonei against multidrug-resistant bacterial strains.
Extracted stem bark using ethanolic maceration and liquid-liquid partitioning for fractionation.
Assessed antibacterial activity using the agar well diffusion method against selected bacterial strains.
Measured zones of inhibition and conducted statistical analysis.
Crude extract showed moderate antibacterial activity with inhibition zones of 12.33 to 13.33 mm.
N-hexane fraction had the strongest inhibition against Staphylococcus aureus (16.67 mm) and Acinetobacter baumannii (14.67 mm).
Ethyl acetate and aqueous fractions had the highest activity against Klebsiella aerogenes (16.67 mm) and Pseudomonas aeruginosa (16.33 mm), respectively.