What is the clinical evidence from this study?

Study design: Other. Intervention: Anti-tropomyosin monoclonal antibodies for microfilament isolation. Primary outcome: Isolation and characterization of tropomyosin-containing microfilaments.

What question did this study set out to answer?

The aim is to isolate and characterize tropomyosin-containing microfilaments from various cultured cell types.

May 1, 1983Journal of Biological ChemistryOpen Access

Isolation and characterization of tropomyosin-containing microfilaments from cultured cells.

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Key result

A new method using anti-tropomyosin monoclonal antibodies successfully isolated tropomyosin-containing microfilaments from various cultured cell types.

Population

Cultured cells including REF-52 cells, L6 myoblasts, 3T3 fibroblasts, Chinese hamster ovary cells, baby…

Design

Preclinical

Authors

FMFumio MatsumuraRutgers, The State University of New Jersey SYShigeko YamashiroRutgers, The State University of New Jersey JLJing‐Jer LinQingdao University of Science and Technology

Discussion

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Implication

Facilitates cytoskeletal research in cell models; leaves open validation in cardiac disease contexts.

Key Points

The aim is to isolate and characterize tropomyosin-containing microfilaments from various cultured cell types.
Developed a method using anti-tropomyosin monoclonal antibodies to induce microfilament bundle formation
Isolated microfilaments by low-speed centrifugation
Conducted electron microscopy and SDS-PAGE analyses for characterization
Isolated microfilaments show 33-34 nm periodic localization of tropomyosin
They activate Mg2+-ATPase activity of myosin similarly to skeletal muscle F-actin
SDS-PAGE revealed five tropomyosin forms and other proteins with Mr values of 83,000 and 37,000

Structured PICO

Population

Cultured cells including REF-52 cells, L6 myoblasts, 3T3 fibroblasts, Chinese hamster ovary cells, baby hamster kidney (BHK-21) cells, mouse neuroblastoma cells, gerbil fibroma cells, and chicken embryo fibroblasts

Intervention

Isolation method using anti-tropomyosin monoclonal antibodies

Outcome

Isolation and characterization of tropomyosin-containing microfilamentssurrogate

A new method using anti-tropomyosin monoclonal antibodies allows for the rapid isolation of functional tropomyosin-containing microfilaments from various cultured cell types.

Cite This Study

Matsumura et al. (1983) studied this question. Anti-tropomyosin monoclonal antibodies for microfilament isolation was evaluated on Isolation and characterization of tropomyosin-containing microfilaments. A new method using anti-tropomyosin monoclonal antibodies successfully isolated tropomyosin-containing microfilaments from various cultured cell types.

synapsesocial.com/papers/6a309949912b1f0ec6bcaefd https://doi.org/10.1016/s0021-9258(18)32460-8

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Isolation and characterization of tropomyosin-containing microfilaments from cultured cells.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Isolation and characterization of tropomyosin-containing microfilaments from cultured cells.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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