What does this research mean for the field?

Viral transformation of rat cultured cells causes a shift in tropomyosin expression at the RNA level, decreasing major tropomyosins and increasing minor ones, which likely contributes to the morphological changes associated with transformation. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

The aim is to analyze the protein composition and expression patterns of tropomyosins in normal versus transformed rat cells.

November 1, 1983Journal of Biological ChemistryOpen Access

Differential expression of tropomyosin forms in the microfilaments isolated from normal and transformed rat cultured cells.

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Population

Normal and transformed rat tissue culture cells, including REF-52, REF-52 transformed by DNA viruses, normal…

Comparison

Transformation by DNA or RNA viruses vs Normal rat culture cells

Design

Preclinical

Authors

FMFumio MatsumuraRutgers, The State University of New Jersey JLJing‐Jer LinQingdao University of Science and Technology SYShigeko YamashiroRutgers, The State University of New Jersey

Discussion

Member takes

Implication

Tropomyosin isoform shifts may drive cytoskeletal changes in transformed cells; leaves open relevance to human tumor progression.

Key Points

The aim is to analyze the protein composition and expression patterns of tropomyosins in normal versus transformed rat cells.
Utilized a novel method for isolating microfilaments from various rat cultured cells.
Compared protein compositions from normal rat cells to those transformed by DNA and RNA viruses.
Conducted cell-free translation of mRNA to assess tropomyosin patterns on two-dimensional gels.
Normal rat cells exhibit three major tropomyosins (Mr = 40,000, 36,500, 32,400) and two minor forms (Mr = 35,000, 32,000).
Transformed cells show decreased levels of major tropomyosins and increased levels of minor tropomyosins.
Changes in tropomyosin expression were linked to morphological alterations in transformed cells.

Structured PICO

Population

Normal and transformed rat tissue culture cells, including REF-52 (established rat embryo cell line), REF-52 transformed by DNA viruses (SV40 or adenovirus type 5), normal rat kidney cells, and normal rat kidney cells transformed by RNA viruses (Kirsten or Rous sarcoma virus)

Intervention

Transformation by DNA or RNA viruses

Comparator

Normal rat culture cells

Outcome

Protein composition of microfilaments (tropomyosin patterns)surrogate

Viral transformation of rat cultured cells alters the pattern of tropomyosin expression at the RNA level, which may be responsible for the rearrangement of stress fibers and morphological transformation.

Cite This Study

Matsumura et al. (1983) studied this question.

synapsesocial.com/papers/6a1df01d79ea62caf6604881 https://doi.org/10.1016/s0021-9258(17)44009-9

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Differential expression of tropomyosin forms in the microfilaments isolated from normal and transformed rat cultured cells.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Differential expression of tropomyosin forms in the microfilaments isolated from normal and transformed rat cultured cells.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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