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The aim is to evaluate the volatile chemical profile of thymol hydrolate and its antimicrobial properties against selected microorganisms.

Thymol Chemotype Thymus vulgaris L. Hydrolate: Volatile Chemical Fraction Composition and Antimicrobial Activity Against Bacterial and Fungal Pathogens

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The aim is to evaluate the volatile chemical profile of thymol hydrolate and its antimicrobial properties against selected microorganisms.
Evaluated antimicrobial activity using disc diffusion and broth microdilution assays.
Analyzed volatile composition using headspace-gas chromatography-mass spectrometry.
Targeted 14 clinically relevant microorganisms including Gram-positive and Gram-negative bacteria, yeasts, and filamentous fungi.
Thymol contributed to 79.9% of the hydrolate composition; linalool was 17.4%.
The hydrolate demonstrated significant activity against Gram-positive bacteria, notably Staphylococcus aureus (MIC 50 = 5.765 mg/mL).
Gram-negative and yeast pathogens showed higher MIC values, with filamentous fungi exhibiting the greatest resistance (Botrytis cinerea MIC 50 = 46.013 mg/mL).

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Evaluates the antimicrobial activity of thymol hydrolate against various pathogens, suggesting its potential as a natural alternative.

Garzoli et al. (2026) studied this question.

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