December 1, 1997Circulation Research
Angiotensin II Induces Apoptosis of Human Endothelial Cells
Key result
Angiotensin II dose-dependently induced apoptosis of human umbilical venous endothelial cells via activation of the caspase cascade, which was completely abrogated by nitric oxide donors.
Population
Human umbilical venous endothelial cells (HUVECs)
Design
Preclinical
Follow-up
18 hours
Authors
Discussion
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Implication
Ang II-driven endothelial apoptosis may contribute to vascular injury; leaves open whether NO donors confer protection in vivo.
Structured PICO
P
Population
In vitro study using human umbilical venous endothelial cells (HUVECs) to investigate Angiotensin II-induced apoptosis.
I
Intervention
Angiotensin II (Ang II) incubation for 18 hours
O
Outcome
Apoptosis of HUVECs measured by ELISA specific for histone-associated DNA fragments and confirmed by DNA laddering and nuclear stainingsurrogate
Angiotensin II induces apoptosis of human endothelial cells via caspase cascade activation, a process that can be inhibited by nitric oxide.
Cite This Study
Dimmeler et al. (1997) studied Endothelial injury in atherosclerosis. Angiotensin II was evaluated on Apoptosis of HUVECs. Angiotensin II dose-dependently induced apoptosis of human umbilical venous endothelial cells via activation of the caspase cascade, which was completely abrogated by nitric oxide donors.