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October 31, 2015Stem Cell Research & TherapyOpen Access

A single intravenous infusion of syngeneic adipose-derived mesenchymal stem cells significantly reduced blood glucose levels and improved glucose tolerance in high-fat diet-induced obese mice.

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Why the study?

Does a single intravenous infusion of syngeneic ASCs improve glucose homeostasis in a high-fat diet-induced obese mouse model?

Population

C57BL/6 mice fed a high-fat diet for 20 weeks to generate the diet-induced obese (DIO) mouse model.

Comparison

Single intravenous infusion of ex vivo expanded… vs DIO or CHOW mice injected with saline.

Design

Preclinical

Follow-up

6 weeks

Key result

A single intravenous infusion of syngeneic adipose-derived mesenchymal stem cells significantly reduced blood glucose levels and improved glucose tolerance in high-fat diet-induced obese mice.

Authors

MCMingjun CaoQPQingjie PanHDHuansheng Dong

Discussion

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Overview

ASC infusion improves glucose control in obese mice; leaves open translation to human metabolic disease.

Structured PICO

Does a single intravenous infusion of syngeneic ASCs improve glucose homeostasis in a high-fat diet-induced obese mouse model?

P
Population
51 male C57BL/6 mice with high-fat diet-induced obesity and insulin resistance, treated with a single infusion of adipose-derived stem cells and followed for 6 weeks.
I
Intervention
Single intravenous infusion of ex vivo expanded syngeneic adipose-derived mesenchymal stem cells (ASCs) at 2 × 10^6 cells per mouse.
C
Comparator
DIO or CHOW mice injected with saline.
O
Outcome
Body weights, blood glucose levels, glucose tolerance, and insulin tolerance at 2 and 6 weeks after cell infusion.surrogate

Main Result

p-value: p=<0.05

A single intravenous infusion of adipose-derived mesenchymal stem cells improves glucose homeostasis, reduces inflammation, and preserves pancreatic beta-cell mass in a mouse model of diet-induced obesity.

Limitations

  • Animal model results may not fully translate to human type 2 diabetes.
  • The study used ASCs from healthy mice; the therapeutic potential of autologous ASCs from obese or insulin-resistant patients remains to be evaluated.

Cite This Study

Cao et al. (2015) studied Diet-induced obesity and insulin resistance (n=51). Syngeneic adipose-derived mesenchymal stem cells (ASCs) vs. Saline injection was evaluated on Blood glucose levels and glucose tolerance (p=<0.05). A single intravenous infusion of syngeneic adipose-derived mesenchymal stem cells significantly reduced blood glucose levels and improved glucose tolerance in high-fat diet-induced obese mice.

synapsesocial.com/papers/6a329e61cf42d9fde8dded38https://doi.org/10.1186/s13287-015-0201-3
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