Lack of Contribution of p66shc to Pressure Overload-Induced Right Heart Hypertrophy

Figures

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  Figure 1. ROS formation in mitochondria isolated from mice three weeks after pulmonary artery banding (PAB) or sham surgery. (A) Basal and (B) rotenone-stimulated reactive oxygen species (ROS) production in right ventricular (RV) mitochondria. ROS formation was measured as the slope of Amplex Ultra Red fluorescence over time in arbitrary units (A.U.). Data are given for wild type (WT) and p66shc knockout (p66KO) mice three weeks after sham surgery (n = 4 and n = 5, respectively) and for WT and p66KO mice three weeks after PAB (n = 6 and n = 5, respectively). Data are expressed as 25%, 50%, and 75% quartiles with whiskers representing the total range. The dots represent the individual data points. *: p < 0.05 vs. WT sham as analyzed by two-way ANOVA.

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  Figure 2. Right ventricular systolic pressure (RVSP) and hypertrophy in mice three weeks after pulmonary artery banding (PAB) or sham surgery. (A) RVSP (in mmHg) and (B) right ventricular weight normalized to the left ventricle and septum weight (RV/LV + S ratio). Data are given for wild type (WT) and p66shc knockout (p66KO) mice three weeks after sham surgery (n = 6 each group) and for WT and p66KO mice three weeks after PAB (n = 7 and n = 9, respectively). Data are expressed as 25%, 50%, and 75% quartiles with whiskers representing the total range. The dots represent the individual data points. *: p < 0.05 vs. WT sham and **: p < 0.05 vs. p66KO sham as analyzed by two-way ANOVA.

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  Figure 3. Cardiac function in mice three weeks after pulmonary artery banding (PAB) or sham surgery. (A) Tricuspid annular plane systolic excursion (TAPSE, in mm) and (B) cardiac index (CI, in ml/min/g BW). Data are given for wild type (WT) and p66shc knockout (p66KO) mice three weeks after sham surgery (n = 6 each group) and for WT and p66KO mice three weeks after PAB (n = 7 and n = 9, respectively). Data are expressed as 25%, 50%, and 75% quartiles with whiskers representing the total range. The dots represent the individual data points. *: p < 0.05 vs. WT sham and **: p < 0.05 vs. p66KO sham as analyzed by two-way ANOVA.

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  Figure 4. Design of the study. In total 31 wild type (WT) and 33 p66shc knockout (p66KO) mice were subjected to pulmonary artery banding (PAB) or sham surgery. Three weeks later, all animals were subjected to an echocardiographic (echo) evaluation. The following day, either the formation of mitochondrial reactive oxygen species (ROS) or cardiomyocyte (CM) function was examined. The mice were further subjected to hemodynamic (hemo) measurements with subsequent determination of the ventricular weights.