Dexamethasone as a Modulator of Renin–Angiotensin System Receptor Expression in Prostate and Ovarian Cancer Cells Under Standard and Low-Serum Conditions

Figures

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  Figure 5. Expression levels of selected genes (AGTR1, AGTR2, LNPEP, MAS1 and NR3C1) in KURAMOCHI, SKOV3, DU145, and PC3 cell lines based on DepMap data. Expression values are shown as log2(TPM + 1). TPM (Transcripts Per Million) represents the normalized abundance of RNA transcripts. The dashed vertical line indicates the median gene expression level within each cell line group.

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  Figure 1. Changes in metabolic activity of SKOV3 and KURAMOCHI ovarian cancer cells following 3- and 9-day DEX treatment (100 nM), expressed as fold change relative to the control and assessed by MTT (A,B) and Alamar Blue (C,D) assays. Data are presented as mean ± SD (n ≥ 3). One-way ANOVA with Tukey’s post hoc test was used for statistical analysis (* p < 0.05).

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  Figure 2. Fold change in colony formation relative to the control in ovarian cancer cells following 3- and 9-day treatment with DEX (100 nM) ((A), SKOV3; (B), KURAMOCHI). Data are presented as mean ± SD (n ≥ 3). Statistical analysis was performed using one-way ANOVA followed by Tukey’s post hoc test (* p < 0.05).

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  Figure 3. Cell cycle distribution (G0/G1, S, and G2/M phases) of SKOV3 and KURAMOCHI ovarian cancer cells following 3- and 9-day treatment with DEX (100 nM). Data are expressed as fold change relative to the control and presented as mean ± SD (n ≥ 3). One-way ANOVA followed by Tukey’s post hoc test was used for statistical analysis (* p < 0.05).

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  Figure 4. Analysis of apoptosis (A) and BCL2/BAX expression (B) in DEX-treated human ovarian cancer cells (100 nM). Apoptosis data are presented as mean ± SEM (n ≥ 3), while gene expression is shown as median fold change relative to control. Arrows indicate statistically significant changes: ↓—decrease; ↑—increase (one-way ANOVA with Tukey’s test or Kruskal–Wallis test with Dunn’s multiple comparisons: * p < 0.05) (LL, live cells; TA, total apoptotic cells).

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  Figure 6. The expression of RAS components genes (AGTR2, AGTR1, MAS1, LNPEP) and the glucocorticoid receptor (NR3C1) were analyzed in prostate and ovarian cancer models. Correlation heatmaps (A) were generated using DepMap data to visualize gene expression patterns in cancer cell lines (SKOV3, KURAMOCHI, DU-145, and PC3), whereas scatter plots of significant relationships (B) were based on patient-derived data obtained from GEPIA.