What question did this study set out to answer?

To examine the regulatory role and mechanism of dexamethasone on angiotensin II receptor subtype expression in cultured rat mesangial cells.

April 1, 1996Hypertension

Regulation of Angiotensin II Receptor Subtypes by Dexamethasone in Rat Mesangial Cells

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Population

Cultured rat mesangial cells

Comparison

Dexamethasone (concentration ≥ 10 nmol/L) vs Untreated cells or co-administration with…

Design

Preclinical

Authors

DCDominique ChanselInserm CLCatherine Llorens‐CortésHeart Failure / Cardiomyopathy SVSophie VandermeerschInserm

Discussion

Member takes

Implication

Provides mechanistic insight into glucocorticoid-AT1 effects in renal cells; leaves open relevance to human hypertension.

Key Points

To examine the regulatory role and mechanism of dexamethasone on angiotensin II receptor subtype expression in cultured rat mesangial cells.
Cultured rat mesangial cells were incubated with dexamethasone at varying times and concentrations, with or without the glucocorticoid receptor antagonist mifepristone.
Radioligand binding assays and inositol phosphate signaling assays evaluated receptor density and signaling function, while RT-PCR and Northern blots quantified AT1A and AT1B receptor mRNA levels.
Dexamethasone reduced cell surface 125I-[Sar1,Ala8]Ang II binding density in a time- and concentration-dependent manner (requiring ≥10 nmol/L and 24 hours) and markedly suppressed Ang II-dependent inositol phosphate accumulation.
Dexamethasone selectively produced a time-dependent decrease in AT1B receptor mRNA beginning at 6 hours, while AT1A receptor mRNA levels remained unchanged.
Co-incubation with mifepristone completely blocked the dexamethasone-induced decreases in both surface receptor binding and AT1B mRNA expression.

Structured PICO

Population

Cultured rat mesangial cells

Intervention

Dexamethasone (concentration ≥ 10 nmol/L)

Comparator

Untreated cells or co-administration with mifepristone

Outcome

Expression of angiotensin II (Ang II) receptors (125I-[Sar1,Ala8]Ang II binding, AT1 receptor density, and AT1B receptor mRNA)surrogate

Dexamethasone downregulates Angiotensin II type 1B receptor mRNA and overall AT1 receptor density in rat mesangial cells, suggesting a mechanism for glucocorticoid-mediated tissue-specific effects.

Cite This Study

Chansel et al. (1996) studied this question.

synapsesocial.com/papers/6a8ad9bdf19228bbb2fb3a70 https://doi.org/10.1161/01.hyp.27.4.867

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Regulation of Angiotensin II Receptor Subtypes by Dexamethasone in Rat Mesangial Cells

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Regulation of Angiotensin II Receptor Subtypes by Dexamethasone in Rat Mesangial Cells

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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