Turmeric extracts nanoparticles ointment for treatment of infected open wounds

To investigate the effects of topical application of turmeric extract nanoparticles ointment in treatment of infected wounds in mice. Thirty male mice had been divided into two groups, G1 (control) and G2 (treated). Two circular (0.5cm) full thicknesses skin wounds were performed on the animal's back. After 24 hours after wounding 0.2 mL of a Pseudomonas aeruginosa bacterial suspension containing 2x108 CFU/mL of bacteria was injected in the right wounds to cause infection. The left wounds weren’t injected or treated and consider as a negative control in the same animal. Wounds of G1 was not treated after receiving a bacterial inoculation. Infected wounds of G2 were treated with a topical application of 3% turmeric extract nanoparticle ointment at a dose of 30mg/cm2 once daily for seven consecutive days. Healing was evaluated by measuring the size of the wound and by histopathology samples taken at 7- and 14-days PI. At the 7th day PI, the size of infected non-treated wounds in G1 (129.35%) was become significantly P < 0.05 larger than the original size, while the treated wounds in G2 were significantly P < 0.05 reduced more than 45.25% in compare with initial wound size. At the 14th day PI the size of infected non-treated wounds in G1 (174.66%) was significantly P < 0.05 larger than the original size, with no healing, while in G2 The reduction in size become significantly (32.36%) smaller. The percentage of wound contraction was significantly increased (P < 0.05) in treated wounds at 7th and 14th days as compared with no wound contraction (0%) in G1. Histopathologically the infected wounds in G1 at 7th and 14th day PI exhibit an absence of epithelial layers, suppurative exudate, dead liquefied tissue, and an abundance of both dead and living neutrophils. G2 at 7th day PI display, no evidence of infection, fully epithelialized epidermis, and immature granulation tissue are visible. On the 14th day PI revealed a well-regenerated epidermis, and mature collagen fibers with myofibroblast in the dermis. The treatment of infected wounds with turmeric extract nanoparticles ointment 3% improved the healing process and accelerated the proliferation, wound contraction, maturation, and remodeling phases of wound healing.