Extended Characterisation of the Coding Sequence of the HLA‐DQB1*02:97 Allele by Next‐ and Third‐Generation Sequencing

Key Points

• The research aims to detail the differences between the HLA-DQB1*02:97 and HLA-DQB1*02:02:01:01 alleles.
• Utilized next-generation and third-generation sequencing techniques
• Analyzed coding sequences in detail
• Focused on single nucleotide variations in exon 4
• Identified a single nucleotide substitution at codon 224 in the HLA-DQB1*02:97 allele
• Differentiated HLA-DQB1*02:97 allele from its counterpart HLA-DQB1*02:02:01:01