Key result
Co-culturing of cardiomyocytes with cardiac fibroblasts leads to orchestrated changes in glucose metabolism enzymes and lactate transport proteins, supporting a cardiac cell-to-cell lactate shuttle.
Why the study?
Resemblance of heart and brain glycolytic profiles prompted the hypothesis that the cell-to-cell lactate shuttle operates in the heart between cardiac fibroblasts and cardiomyocytes.
Population
Cardiomyocytes and cardiac fibroblasts
Comparison
Co-culturing of cardiomyocytes with cardiac fibroblasts
Design
In vitro co-culture study
Authors
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Supports cardiac lactate shuttle hypothesis in vitro; hypothesis-generating and requires clinical trials before informing heart failure therapies.
Cardiomyocytes and cardiac fibroblasts form a metabolic syncytium via a cell-to-cell lactate shuttle mediated by microvesicles, providing a theoretical rationale for novel therapeutic strategies in age-related heart failure.
Gizak et al. (2020) studied Cardiac metabolism. Co-culturing of cardiomyocytes with cardiac fibroblasts vs. Monocultures of cardiomyocytes or fibroblasts was evaluated on Changes in expression and localization of glucose metabolism enzymes and lactate transport proteins. Co-culturing of cardiomyocytes with cardiac fibroblasts leads to orchestrated changes in glucose metabolism enzymes and lactate transport proteins, supporting a cardiac cell-to-cell lactate shuttle.
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