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March 23, 1999CirculationOpen Access

Tissue Expression and Immunolocalization of Tumor Necrosis Factor-α in Postinfarction Dysfunctional Myocardium

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Population

Male rats in a model of large myocardial infarction

Design

Preclinical, randomized to proximal left anterior descending ligation

Follow-up

up to 35 days

Key result

In a rat model of myocardial infarction, TNF-alpha protein levels rose 8- to 10-fold above normal in the infarct and peri-infarct zones and 4- to 5-fold in the contralateral zone.

Authors

MIMin W. IrwinSMSusanna MakDMDouglas L. Mann

Discussion

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Overview

TNF-α expression beyond infarct zones broadens post-MI remodeling targets; leaves open human therapeutic relevance.

Study Design

Type

RCT

Randomization

randomized

Structured PICO

P
Population
Male rats subjected to proximal left anterior descending ligation to model large myocardial infarction, followed for up to 35 days.
I
Intervention
Proximal left anterior descending ligation
O
Outcome
Gene expression and protein production of TNF-alpha and TNF-R1/R2 from the infarct, peri-infarct, and contralateral zones of infarcted heartsurrogate

TNF-alpha production forms an important intrinsic myocardial stress response to injury, with expression occurring early and sustained in both infarcted and non-infarcted myocardium.

Cite This Study

Irwin et al. (1999) conducted an RCT in myocardial infarction. proximal left anterior descending ligation vs. normal levels was evaluated on gene expression and protein production of TNF-alpha and TNF-R1/R2. In a rat model of myocardial infarction, TNF-alpha protein levels rose 8- to 10-fold above normal in the infarct and peri-infarct zones and 4- to 5-fold in the contralateral zone.

synapsesocial.com/papers/6a4af364321fc44b1362b446https://doi.org/10.1161/01.cir.99.11.1492
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